Detailed information of TA system TA228730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 720931..721580
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67241
• Locus tag: LVJ68_RS03295
• Protein ID: WP_003403236.1
• Coordinates: 721185..721580 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ34
• Locus tag: LVJ68_RS03290
• Protein ID: WP_003403235.1
• Coordinates: 720931..721185 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS03265 (LVJ68_03260)	716057..716140	+	84	Protein_647	galactose-1-phosphate uridylyltransferase	-
LVJ68_RS03270 (LVJ68_03265)	716159..717240	+	1082	Protein_648	galactose-1-phosphate uridylyltransferase	-
LVJ68_RS03275 (LVJ68_03270)	717237..718328	+	1092	WP_003906403.1	galactokinase	-
LVJ68_RS03280 (LVJ68_03275)	718723..719787	+	1065	WP_003898532.1	zinc ribbon domain-containing protein	-
LVJ68_RS03285 (LVJ68_03280)	719891..720838	+	948	WP_003403232.1	DUF732 domain-containing protein	-
LVJ68_RS03290 (LVJ68_03285)	720931..721185	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	Antitoxin
LVJ68_RS03295 (LVJ68_03290)	721185..721580	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	Toxin
LVJ68_RS03300 (LVJ68_03295)	721674..722414	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
LVJ68_RS03305 (LVJ68_03300)	722546..722806	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ68_RS03310 (LVJ68_03305)	722803..723210	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	-
LVJ68_RS03315 (LVJ68_03310)	723282..724433	-	1152	WP_003403248.1	FIST N-terminal domain-containing protein	-
LVJ68_RS03320 (LVJ68_03315)	724526..726253	-	1728	WP_070892740.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14250.04 Da
• Isoelectric Point: 4.7475

>T228730 WP_003403236.1 NZ_CP089773:721185-721580 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Nucleotide

• Length: 396 bp

>T228730 NZ_CP124339:c3603871-3603569 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9139.52 Da
• Isoelectric Point: 9.2702

>AT228730 WP_003403235.1 NZ_CP089773:720931-721185 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Nucleotide

• Length: 255 bp

>AT228730 NZ_CP124339:c3603533-3603192 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• PDB: 4XGR
• PDB: 4XGQ

Antitoxin

• AlphaFold DB: A0A7U4BSC2