Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 720931..721580 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag LVJ68_RS03295 Protein ID WP_003403236.1
Coordinates 721185..721580 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag LVJ68_RS03290 Protein ID WP_003403235.1
Coordinates 720931..721185 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS03265 (LVJ68_03260) 716057..716140 + 84 Protein_647 galactose-1-phosphate uridylyltransferase -
LVJ68_RS03270 (LVJ68_03265) 716159..717240 + 1082 Protein_648 galactose-1-phosphate uridylyltransferase -
LVJ68_RS03275 (LVJ68_03270) 717237..718328 + 1092 WP_003906403.1 galactokinase -
LVJ68_RS03280 (LVJ68_03275) 718723..719787 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ68_RS03285 (LVJ68_03280) 719891..720838 + 948 WP_003403232.1 DUF732 domain-containing protein -
LVJ68_RS03290 (LVJ68_03285) 720931..721185 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
LVJ68_RS03295 (LVJ68_03290) 721185..721580 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
LVJ68_RS03300 (LVJ68_03295) 721674..722414 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
LVJ68_RS03305 (LVJ68_03300) 722546..722806 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ68_RS03310 (LVJ68_03305) 722803..723210 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
LVJ68_RS03315 (LVJ68_03310) 723282..724433 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
LVJ68_RS03320 (LVJ68_03315) 724526..726253 - 1728 WP_070892740.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T228730 WP_003403236.1 NZ_CP089773:721185-721580 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T228730 NZ_CP124339:c3603871-3603569 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT228730 WP_003403235.1 NZ_CP089773:720931-721185 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT228730 NZ_CP124339:c3603533-3603192 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References