228644

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1047088..1047713 Replicon chromosome
Accession NZ_CP089771
Organism Klebsiella pneumoniae strain HKE12

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LVO50_RS05100 Protein ID WP_002882817.1
Coordinates 1047330..1047713 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LVO50_RS05095 Protein ID WP_004150355.1
Coordinates 1047088..1047330 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO50_RS05070 (1043078) 1043078..1043677 + 600 WP_004151865.1 glucose-1-phosphatase -
LVO50_RS05075 (1043671) 1043671..1044531 + 861 WP_004146232.1 virulence factor BrkB family protein -
LVO50_RS05080 (1044528) 1044528..1044965 + 438 WP_023302745.1 D-aminoacyl-tRNA deacylase -
LVO50_RS05085 (1045010) 1045010..1045951 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LVO50_RS05090 (1045966) 1045966..1046883 - 918 WP_004213381.1 alpha/beta hydrolase -
LVO50_RS05095 (1047088) 1047088..1047330 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LVO50_RS05100 (1047330) 1047330..1047713 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LVO50_RS05105 (1047887) 1047887..1048816 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LVO50_RS05110 (1048813) 1048813..1049448 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LVO50_RS05115 (1049445) 1049445..1050347 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T228644 WP_002882817.1 NZ_CP089771:1047330-1047713 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T228644 NZ_CP124330:60172-60321 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT228644 WP_004150355.1 NZ_CP089771:1047088-1047330 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT228644 NZ_CP124330:c60127-60068 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References