Detailed information of TA system TA228495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3192423..3193110
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: LVJ91_RS15215
• Protein ID: WP_003414624.1
• Coordinates: 3192667..3193110 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: LVJ91_RS15210
• Protein ID: WP_003414620.1
• Coordinates: 3192423..3192680 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS15190 (LVJ91_15195)	3187743..3188597	-	855	WP_070894445.1	GNAT family N-acetyltransferase	-
LVJ91_RS15195 (LVJ91_15200)	3188653..3189816	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
LVJ91_RS15200 (LVJ91_15205)	3189833..3191047	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
LVJ91_RS15205 (LVJ91_15210)	3191055..3192296	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
LVJ91_RS15210 (LVJ91_15215)	3192423..3192680	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
LVJ91_RS15215 (LVJ91_15220)	3192667..3193110	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS15220 (LVJ91_15225)	3193190..3193852	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
LVJ91_RS15225 (LVJ91_15230)	3193948..3194139	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
LVJ91_RS15230 (LVJ91_15235)	3194471..3196219	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
LVJ91_RS15235 (LVJ91_15240)	3196315..3196896	+	582	WP_003414644.1	fasciclin domain-containing protein	-
LVJ91_RS15240 (LVJ91_15245)	3196996..3197262	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T228495 WP_003414624.1 NZ_CP089610:3192667-3193110 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T228495 NZ_CP123992:16109-16465 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT228495 WP_003414620.1 NZ_CP089610:3192423-3192680 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT228495 NZ_CP123992:16458-16760 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0