Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3079954..3080633 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag LVJ91_RS14610 Protein ID WP_003414059.1
Coordinates 3079954..3080370 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag LVJ91_RS14615 Protein ID WP_003414061.1
Coordinates 3080367..3080633 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS14590 (LVJ91_14595) 3076006..3076908 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
LVJ91_RS14595 (LVJ91_14600) 3076977..3077729 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
LVJ91_RS14600 (LVJ91_14610) 3077973..3078248 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
LVJ91_RS14605 (LVJ91_14615) 3078245..3079867 - 1623 WP_003906897.1 class I SAM-dependent DNA methyltransferase -
LVJ91_RS14610 (LVJ91_14620) 3079954..3080370 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
LVJ91_RS14615 (LVJ91_14625) 3080367..3080633 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ91_RS14620 (LVJ91_14630) 3080659..3081054 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
LVJ91_RS14625 (LVJ91_14635) 3081051..3081320 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ91_RS14630 (LVJ91_14640) 3081330..3082424 - 1095 WP_003414068.1 restriction endonuclease subunit S -
LVJ91_RS14635 (LVJ91_14645) 3082421..3082840 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
LVJ91_RS14640 (LVJ91_14650) 3082839..3082913 + 75 Protein_2889 hypothetical protein -
LVJ91_RS14645 (LVJ91_14655) 3082914..3083393 - 480 WP_003414073.1 dihydrofolate reductase -
LVJ91_RS14650 (LVJ91_14660) 3083464..3084264 - 801 WP_009939104.1 thymidylate synthase -
LVJ91_RS14655 (LVJ91_14665) 3084420..3085157 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T228489 WP_003414059.1 NZ_CP089610:c3080370-3079954 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T228489 NZ_CP123983:885030-885395 [Staphylococcus haemolyticus]
ATGATTAGAAGAGGCGATGTATATTTAGCTGATTTGTCACCAGTACAGGGCTCAGAACAAGGGGGAGTCAGACCTGTAGT
TATCATTCAAAACGATACTGGTAACAAATATAGTCCTACCGTAATCGTTGCTGCGATTACGGGAAGGATTAATAAAGCGA
AAATACCCACACATGTTGAAATCGAGAAAAAGAAATACAGACTTGATAAAGATTCAGTTATTCTACTCGAGCAAATTAGA
ACATTAGATAAAAAACGACTAAAAGAAAAATTAACATATCTATCTGATGAGAAAATGCAAGAAGTGGATGAAGCGTTAGA
CATTAGTCTAGGATTGCATGATGAAGTTAAAACGCAAAACACATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT228489 WP_003414061.1 NZ_CP089610:c3080633-3080367 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT228489 NZ_CP123983:884863-885033 [Staphylococcus haemolyticus]
ATGCTTACTTTCAATCAAAATAGAAGTCACAGTCTTGAACAATCTTTAAAAGAAGGCTATGCACAAATGGCCGAATTAAA
TCTCTCCCTAGCGACAGAAGCTTTTCCTCTAGAATGCGAAGCTTGTGATTGCAACGAAACGTACCTTATTTCTAATTATA
AAAGTGAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References