Detailed information of TA system TA228481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2413419..2413948
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: LVJ91_RS11295
• Protein ID: WP_003411124.1
• Coordinates: 2413419..2413736 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: LVJ91_RS11300
• Protein ID: WP_003411127.1
• Coordinates: 2413733..2413948 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS11265 (LVJ91_11280)	2408556..2409632	+	1077	WP_003411110.1	hypothetical protein	-
LVJ91_RS11270 (LVJ91_11285)	2409629..2409910	+	282	WP_003411112.1	DUF5703 family protein	-
LVJ91_RS11275 (LVJ91_11290)	2409946..2411019	+	1074	WP_003411116.1	quinone-dependent dihydroorotate dehydrogenase	-
LVJ91_RS11280 (LVJ91_11295)	2411024..2411554	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
LVJ91_RS11285 (LVJ91_11300)	2411602..2412948	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
LVJ91_RS11295 (LVJ91_11310)	2413419..2413736	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ91_RS11300 (LVJ91_11315)	2413733..2413948	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
LVJ91_RS11305 (LVJ91_11320)	2414203..2415261	+	1059	WP_003411129.1	phosphoribosyltransferase family protein	-
LVJ91_RS11310 (LVJ91_11325)	2415391..2415747	-	357	WP_003411130.1	hypothetical protein	-
LVJ91_RS11315 (LVJ91_11330)	2415842..2416624	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
LVJ91_RS11320 (LVJ91_11335)	2416892..2417182	-	291	WP_003900476.1	YggT family protein	-
LVJ91_RS11325 (LVJ91_11340)	2417344..2418000	-	657	WP_003411133.1	cell division protein SepF	-
LVJ91_RS11330 (LVJ91_11345)	2418066..2418842	-	777	WP_003411137.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T228481 WP_003411124.1 NZ_CP089610:c2413736-2413419 [Mycobacterium tuberculosis]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T228481 NZ_CP123976:76256-76651 [Thioclava nitratireducens]
ATGAGCGCTGAATTCGCAGACACGAATGTTGTACTCTACCTGCTCGACGATGGTGCAAAGGCTGAGAGGGCAGAGGAGAT
CTTGGGGCAGGGACCCCGGGTCAGCGTCCAAGTCCTGAACGAAACGATGGTAAATTGCCGGCGCAAAGCTGGGCTCAGTT
GGGAAGATACCGGAGCAATTCTTGAGGCTATCCAGTCTCTTTGCCCGGTCGAGGACCTGACGGTTCAAACCCATCAAGTC
GGTCGCGCGTTGGCAGAGAAGTACCAGTTATCGGTCTATGACGCGATGATCGTGTCTGCCGCGCTGATCGCTGGGTGCAC
GACGTTGTGGACTGAGGACACGCACGACGGATTGCTGGTCGAGGATCGGCTGCGCATCGTCAATCCATTTGCCTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT228481 WP_003411127.1 NZ_CP089610:c2413948-2413733 [Mycobacterium tuberculosis]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT228481 NZ_CP123976:76039-76266 [Thioclava nitratireducens]
ATGCAAGTTGCCAAATGGGGAAACTCCCTTGCAGTCCGGCTACCCGCGGATCTTGTGCGGGAACTCGGCTTGAAGGAAGG
CGACCAGGTCGATCTGGTAAAGGACGACGGAACCATTCTTGTCAGGCGCCAACCTCGCGCCGATGAAGTCCTGCAAGGGC
TAAGGCGCTTTCGCGGAAAACTGCCCAAGGACGCGCGTCTAAGCCGCGACGCCGCGCATGAGCGCTGA

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4