Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2333295..2333931 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag LVJ91_RS10890 Protein ID WP_003410654.1
Coordinates 2333521..2333931 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag LVJ91_RS10885 Protein ID WP_003410651.1
Coordinates 2333295..2333528 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS10870 (LVJ91_10885) 2328752..2329144 + 393 WP_229303073.1 metal ABC transporter permease -
LVJ91_RS10875 (LVJ91_10890) 2329145..2329549 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
LVJ91_RS10880 (LVJ91_10895) 2329633..2333217 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
LVJ91_RS10885 (LVJ91_10900) 2333295..2333528 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
LVJ91_RS10890 (LVJ91_10905) 2333521..2333931 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
LVJ91_RS10895 (LVJ91_10910) 2333915..2335006 + 1092 WP_003900454.1 precorrin-3B synthase -
LVJ91_RS10900 (LVJ91_10915) 2335016..2335642 + 627 WP_003410658.1 precorrin-8X methylmutase -
LVJ91_RS10905 (LVJ91_10920) 2335639..2337165 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
LVJ91_RS10910 (LVJ91_10925) 2337111..2338334 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T228479 WP_003410654.1 NZ_CP089610:2333521-2333931 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T228479 NZ_CP123973:148884-149075 [Ligilactobacillus salivarius]
ATGCCACTTACAGGAAAAGATATGGTCAAACTATTACTGGATAATGGTTTTGTTGAACGCAGGGTAAATGGATCCCATCA
CCAATTATATAAAGACGGTGTGCGTATCACAGTTCCAATACACGGTAATCAAGATTTGGGCAAAGGGCTAGAACGTAAAA
TTCTAAAAGAAGCTGGAATTGATAAAAAATAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT228479 WP_003410651.1 NZ_CP089610:2333295-2333528 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT228479 NZ_CP123973:149136-149540 [Ligilactobacillus salivarius]
ATGAGTAACAAGCAAAAAATGGTAGTTGCTTATCCAGCTATATTCACACCAGAAGAAAATGGTGGTTATTTAATTGAATT
TCCAGATATACAAGGTGCATTTACTGGAATTAATAATGACGATTTAGGATATGGTATGGAAATGGCAAGCGAAGTGTTAG
GATTAACCGTAGCTGATTATTTAGAAAGTGGCGATGACTTAAGTAAACCTACAGCAGTTAACAAGGTTAAACATAAAGAA
GGCTCTTTCGTTACACTAGTAAGCACAGATGTTAGTAAGTATTTAGATAATCAAAAACTAGTCAAAAAGACATTAACTAT
ACCTAAATGGGCTAATCAAAAGGCAATCAAAGAAAACATTAATTTTTCAGCATTATTGACTAAAGCAATCCTTGACTATA
ATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References