Detailed information of TA system TA228463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1389137..1389825
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ91_RS06625
• Protein ID: WP_003406304.1
• Coordinates: 1389394..1389825 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ91_RS06620
• Protein ID: WP_003406302.1
• Coordinates: 1389137..1389397 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS06600 (LVJ91_06595)	1384714..1385538	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LVJ91_RS06605 (LVJ91_06600)	1385543..1386724	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ91_RS06610 (LVJ91_06605)	1386801..1387901	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ91_RS06615 (LVJ91_06610)	1388072..1389061	+	990	WP_003406301.1	malate dehydrogenase	-
LVJ91_RS06620 (LVJ91_06615)	1389137..1389397	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ91_RS06625 (LVJ91_06620)	1389394..1389825	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS06630 (LVJ91_06625)	1389848..1391536	-	1689	WP_003910308.1	PE family protein	-
LVJ91_RS06635 (LVJ91_06630)	1391716..1392576	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ91_RS06640 (LVJ91_06635)	1392657..1393487	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ91_RS06645 (LVJ91_06640)	1393544..1393837	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ91_RS06650 (LVJ91_06645)	1393834..1394103	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T228463 WP_003406304.1 NZ_CP089610:1389394-1389825 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T228463 NZ_CP123963:979752-980087 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT228463 WP_003406302.1 NZ_CP089610:1389137-1389397 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT228463 NZ_CP123963:979504-979752 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6