Detailed information of TA system TA228462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244444..1245012
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ91_RS05950
• Protein ID: WP_003405865.1
• Coordinates: 1244638..1245012 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ91_RS05945
• Protein ID: WP_003405863.1
• Coordinates: 1244444..1244641 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS05925 (LVJ91_05925)	1240485..1241123	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ91_RS05930 (LVJ91_05930)	1241213..1242220	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ91_RS05935 (LVJ91_05935)	1242237..1243220	-	984	WP_003898731.1	hypothetical protein	-
LVJ91_RS05940 (LVJ91_05940)	1243283..1244356	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ91_RS05945 (LVJ91_05945)	1244444..1244641	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ91_RS05950 (LVJ91_05950)	1244638..1245012	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS05955 (LVJ91_05955)	1245215..1245913	+	699	WP_003898733.1	hypothetical protein	-
LVJ91_RS05960 (LVJ91_05960)	1246031..1246216	+	186	WP_003901093.1	hypothetical protein	-
LVJ91_RS05965 (LVJ91_05965)	1246143..1246418	-	276	WP_003405867.1	hypothetical protein	-
LVJ91_RS05970 (LVJ91_05970)	1246661..1246984	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ91_RS05975 (LVJ91_05975)	1246999..1247697	-	699	WP_031646953.1	hypothetical protein	-
LVJ91_RS05980 (LVJ91_05980)	1247731..1247940	+	210	WP_003911400.1	hypothetical protein	-
LVJ91_RS05985 (LVJ91_05985)	1247892..1248662	-	771	Protein_1179	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T228462 WP_003405865.1 NZ_CP089610:1244638-1245012 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T228462 NZ_CP123963:841236-841643 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT228462 WP_003405863.1 NZ_CP089610:1244444-1244641 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT228462 NZ_CP123963:840989-841255 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07