Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 713939..714564 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name vapC Uniprot ID G0TQA0
Locus tag LVJ91_RS03250 Protein ID WP_003403218.1
Coordinates 714163..714564 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ36
Locus tag LVJ91_RS03245 Protein ID WP_003403213.1
Coordinates 713939..714166 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS03220 (LVJ91_03215) 709118..709339 - 222 WP_003898525.1 DUF4926 domain-containing protein -
LVJ91_RS03225 (LVJ91_03220) 709481..710086 + 606 WP_003898526.1 hypothetical protein -
LVJ91_RS03230 (LVJ91_03225) 710105..712672 - 2568 WP_009937370.1 SEC-C metal-binding domain-containing protein -
LVJ91_RS03235 (LVJ91_03230) 712756..713505 + 750 WP_003898528.1 hypothetical protein -
LVJ91_RS03240 (LVJ91_03235) 713502..713744 + 243 WP_003403210.1 hypothetical protein -
LVJ91_RS03245 (LVJ91_03240) 713939..714166 + 228 WP_003403213.1 antitoxin VapB29 Antitoxin
LVJ91_RS03250 (LVJ91_03245) 714163..714564 + 402 WP_003403218.1 type II toxin-antitoxin system ribonuclease VapC29 Toxin
LVJ91_RS03255 (LVJ91_03250) 714693..714776 + 84 Protein_645 galactose-1-phosphate uridylyltransferase -
LVJ91_RS03260 (LVJ91_03255) 714795..715876 + 1082 Protein_646 galactose-1-phosphate uridylyltransferase -
LVJ91_RS03265 (LVJ91_03260) 715873..716964 + 1092 WP_057951135.1 galactokinase -
LVJ91_RS03270 (LVJ91_03265) 717359..718423 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ91_RS03275 (LVJ91_03270) 718527..719474 + 948 WP_003403232.1 DUF732 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-120)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 13937.76 Da        Isoelectric Point: 5.7441

>T228450 WP_003403218.1 NZ_CP089610:714163-714564 [Mycobacterium tuberculosis]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLIPTTT

Download         Length: 402 bp

>T228450 NZ_CP123958:c728176-727907 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8163.17 Da        Isoelectric Point: 5.6307

>AT228450 WP_003403213.1 NZ_CP089610:713939-714166 [Mycobacterium tuberculosis]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Download         Length: 228 bp

>AT228450 NZ_CP123958:c728429-728163 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQA0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSC9

References