Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5176667..5177292 Replicon chromosome
Accession NZ_CP089509
Organism Klebsiella pneumoniae strain GD21SC417

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag LT986_RS25360 Protein ID WP_004187928.1
Coordinates 5176667..5177050 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LT986_RS25365 Protein ID WP_004150355.1
Coordinates 5177050..5177292 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT986_RS25345 (5174033) 5174033..5174935 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LT986_RS25350 (5174932) 5174932..5175567 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LT986_RS25355 (5175564) 5175564..5176493 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LT986_RS25360 (5176667) 5176667..5177050 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
LT986_RS25365 (5177050) 5177050..5177292 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LT986_RS25370 (5177486) 5177486..5178403 + 918 WP_105910615.1 alpha/beta hydrolase -
LT986_RS25375 (5178417) 5178417..5179358 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LT986_RS25380 (5179403) 5179403..5179840 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LT986_RS25385 (5179837) 5179837..5180697 - 861 WP_004146232.1 virulence factor BrkB family protein -
LT986_RS25390 (5180691) 5180691..5181290 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T228344 WP_004187928.1 NZ_CP089509:c5177050-5176667 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T228344 NZ_CP123843:c325965-325696 [Agrobacterium fabrum]
TTGATCTGGAGAATTGAATTCCAGCGAAATGCCGTCAAGCAACTGGGCAAGCTGACGAAGAGCGATGCCAATCGCATCGT
TTCATTTCTGACGGACCGCGTTGCCCAGGACGACAATCCGCGCAGAGCCGGCGCAGCGCTGCAAGGTTCCGAGCTCGGAA
ATTTCTGGCATTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGACCACAAGCTGGTCGTTCTGGTGGTCGAA
ATCGGCCATCGCCGTGAAATTTACCGTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT228344 WP_004150355.1 NZ_CP089509:c5177292-5177050 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT228344 NZ_CP123843:c326182-325949 [Agrobacterium fabrum]
ATGAGCAAACAGACGGCCATTCGCCTGCCGGACGAGACCTATGAGCGGCTGAAGGCGCTGTCTGAGCGGACCGGCCGCAC
ATCCGCCTATTACATCCGCGAGGCGATCGAGAAGCATATCGAGGATATGGAAGACCTCTATCTTGCCGAAGAGGCGATGC
GGAAGCACATCGCCAATAAGGAGCGCACCTATACTCTGGAAGAAGTGATGCGTGATCTTGATCTGGAGAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References