Detailed information of TA system TA228276

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 603095..603930
• Replicon: chromosome
• Accession: NZ_CP089445
• Organism: Escherichia coli strain 5-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: LT922_RS02900
• Protein ID: WP_000854759.1
• Coordinates: 603553..603930 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: LT922_RS02895
• Protein ID: WP_001295723.1
• Coordinates: 603095..603463 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT922_RS02870 (600210)	600210..600405	+	196	Protein_555	DUF905 family protein	-
LT922_RS02875 (600523)	600523..601341	+	819	WP_001234738.1	DUF932 domain-containing protein	-
LT922_RS02880 (601683)	601683..602156	+	474	WP_001350782.1	antirestriction protein	-
LT922_RS02885 (602172)	602172..602648	+	477	WP_001186775.1	RadC family protein	-
LT922_RS02890 (602711)	602711..602932	+	222	WP_000692345.1	DUF987 domain-containing protein	-
LT922_RS02895 (603095)	603095..603463	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LT922_RS02900 (603553)	603553..603930	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
LT922_RS02905 (603927)	603927..604415	+	489	WP_000761690.1	DUF5983 family protein	-
LT922_RS02910 (604432)	604432..604608	+	177	WP_000839286.1	DUF957 domain-containing protein	-
LT922_RS02915 (604714)	604714..604863	+	150	Protein_564	hypothetical protein	-
LT922_RS02920 (605230)	605230..605406	+	177	Protein_565	helix-turn-helix domain-containing protein	-
LT922_RS02925 (606197)	606197..607819	+	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	589400..624401	35001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T228276 WP_000854759.1 NZ_CP089445:603553-603930 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T228276 NZ_CP123607:1524804-1525157 [Serratia marcescens]
GTGTGGGAAATTAAAACAACGGATGCATTTGATCATTGGTTCAGATCACTCAGCGATGCCGACCGGGCCTGCGTGCTTGC
TGCATTGATGGTCCTGCGGGAAAAAGGACCCGGCTTGCCCAGGCCCTATGCCGATACGGTTAAAGGCTCGCGTTACAGCA
ACATGAAAGAGCTGCGTATTCAGAGCCGTGGCGAACCGTTGCGGGCATTTTTCGCCTTCGATCCGTACCGTATCGGCATG
GTGCTTTGCGCGGGCAATAAAGCGGGCGATGAAAAACGTTTTTACGATCGAATGCTGCAGATTGCCGATCGGGAATTCAC
GAATTGGCTGAACACACTAAAAGAGAAGGAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT228276 WP_001295723.1 NZ_CP089445:603095-603463 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT228276 NZ_CP123607:1525162-1525464 [Serratia marcescens]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGAGGAAAAGCCCGAGGTCGTGGCCGAAGCGCAGGCAATGGCGACGGACAT
TATGCTGAACATCCATCTTGCCGAGCTGCGCGAGAAGGTGCAGAAAACCCAGGTTGAAATGGCGCAGGCGCTGGGTATCA
GGCAACCGACCGTCGCCGTGATGGAAAAACCGGGCCGCGATCTGAAACTGTCTTCGCTCAAACGCTATGTTGAGGCGGTG
GGCGGCAAACTGCGCCTGGATGTCGAACTGCCGGACGGTTCGCACTACGAATTTGTGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52