Detailed information of TA system TA228272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 226793..227544
• Replicon: plasmid pHNLW22-1
• Accession: NZ_CP089442
• Organism: Klebsiella quasipneumoniae strain GLW9C22

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LT990_RS25835
• Protein ID: WP_072046196.1
• Coordinates: 226793..227275 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: LT990_RS25840
• Protein ID: WP_004902250.1
• Coordinates: 227266..227544 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT990_RS25820 (LT990_25815)	222070..223596	-	1527	WP_250681185.1	EAL domain-containing protein	-
LT990_RS25825 (LT990_25820)	224004..224930	+	927	WP_250681186.1	IS5-like element IS903B family transposase	-
LT990_RS25830 (LT990_25825)	225218..225757	-	540	WP_135720529.1	hypothetical protein	-
LT990_RS25835 (LT990_25830)	226793..227275	-	483	WP_072046196.1	GNAT family N-acetyltransferase	Toxin
LT990_RS25840 (LT990_25835)	227266..227544	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
LT990_RS25845 (LT990_25840)	227663..227875	-	213	WP_004902255.1	hypothetical protein	-
LT990_RS25850 (LT990_25845)	227983..228324	-	342	WP_135720527.1	hypothetical protein	-
LT990_RS26255	228910..229056	-	147	Protein_234	DUF2235 domain-containing protein	-
LT990_RS25860 (LT990_25855)	229091..229201	+	111	Protein_235	glutathione ABC transporter permease GsiC	-
LT990_RS25865 (LT990_25860)	229285..230955	-	1671	WP_032413257.1	AMP-binding protein	-
LT990_RS25870 (LT990_25865)	230936..232201	-	1266	WP_048281137.1	MSMEG_0569 family flavin-dependent oxidoreductase	-
LT990_RS25875 (LT990_25870)	232219..232509	-	291	WP_032413253.1	MSMEG_0570 family nitrogen starvation response protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / aadA2 / dfrA12 / tet(A)	pla	1..259761	259761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17678.45 Da
• Isoelectric Point: 8.9130

>T228272 WP_072046196.1 NZ_CP089442:c227275-226793 [Klebsiella quasipneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWSGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T228272 NZ_CP123600:c24497-24081 [Enterobacter cloacae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT228272 WP_004902250.1 NZ_CP089442:c227544-227266 [Klebsiella quasipneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT228272 NZ_CP123600:c24724-24494 [Enterobacter cloacae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTAG
ACAGGGCCGATCCGGACTTTATGGCGCAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3