Detailed information of TA system TA228271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 202328..203055
• Replicon: plasmid pHNLW22-1
• Accession: NZ_CP089442
• Organism: Klebsiella quasipneumoniae strain GLW9C22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LT990_RS25715
• Protein ID: WP_011251285.1
• Coordinates: 202744..203055 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LT990_RS25710
• Protein ID: WP_011251286.1
• Coordinates: 202328..202747 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT990_RS25690 (LT990_25685)	198019..198576	-	558	WP_017900872.1	HutD family protein	-
LT990_RS25695 (LT990_25690)	198573..199334	-	762	WP_017900871.1	histidine utilization repressor	-
LT990_RS25700 (LT990_25695)	199437..200804	+	1368	WP_023329010.1	formimidoylglutamate deiminase	-
LT990_RS25705 (LT990_25700)	201213..202181	-	969	Protein_204	IS5 family transposase	-
LT990_RS25710 (LT990_25705)	202328..202747	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LT990_RS25715 (LT990_25710)	202744..203055	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LT990_RS25720 (LT990_25715)	203260..203697	-	438	Protein_207	DDE-type integrase/transposase/recombinase	-
LT990_RS25725 (LT990_25720)	203811..204149	+	339	WP_023288266.1	transposase	-
LT990_RS25730 (LT990_25725)	204146..204493	+	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
LT990_RS25735 (LT990_25730)	204542..205591	+	1050	Protein_210	IS66 family transposase	-
LT990_RS25740 (LT990_25735)	205649..206616	-	968	Protein_211	IS5 family transposase	-
LT990_RS25745 (LT990_25740)	206660..207097	+	438	WP_135720512.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / aadA2 / dfrA12 / tet(A)	pla	1..259761	259761
	-	inside	IScluster/Tn	-	-	201213..212838	11625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T228271 WP_011251285.1 NZ_CP089442:c203055-202744 [Klebsiella quasipneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T228271 NZ_CP123600:9813-10295 [Enterobacter cloacae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTGCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT228271 WP_011251286.1 NZ_CP089442:c202747-202328 [Klebsiella quasipneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT228271 NZ_CP123600:9559-9825 [Enterobacter cloacae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1