Detailed information of TA system TA022827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2324336..2324964
• Replicon: chromosome
• Accession: NC_010602
• Organism: Leptospira biflexa serovar Patoc strain Patoc 1 (Paris)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0STA6
• Locus tag: LEPBI_RS11095
• Protein ID: WP_012389212.1
• Coordinates: 2324566..2324964 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0STA5
• Locus tag: LEPBI_RS11090
• Protein ID: WP_012389211.1
• Coordinates: 2324336..2324566 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEPBI_RS11055	2319352..2320029	+	678	WP_012389204.1	YdcF family protein	-
LEPBI_RS11060	2320539..2320751	+	213	WP_012389205.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LEPBI_RS11065	2320752..2321150	+	399	WP_012389206.1	type II toxin-antitoxin system death-on-curing family toxin	-
LEPBI_RS11070	2321346..2322224	+	879	WP_012389207.1	hypothetical protein	-
LEPBI_RS11080	2323392..2323637	+	246	WP_012389209.1	ribbon-helix-helix protein, CopG family	-
LEPBI_RS11085	2323637..2324053	+	417	WP_012389210.1	putative toxin-antitoxin system toxin component, PIN family	-
LEPBI_RS11090	2324336..2324566	+	231	WP_012389211.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LEPBI_RS11095	2324566..2324964	+	399	WP_012389212.1	type II toxin-antitoxin system VapC family toxin	Toxin
LEPBI_RS11100	2325235..2325612	+	378	WP_012389213.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LEPBI_RS11105	2325578..2325895	+	318	WP_012476326.1	helix-turn-helix transcriptional regulator	-
LEPBI_RS11110	2326106..2326771	+	666	WP_012389215.1	hydrolase	-
LEPBI_RS11115	2327227..2327754	+	528	WP_012389217.1	hypothetical protein	-
LEPBI_RS11125	2329164..2329445	+	282	WP_012476327.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LEPBI_RS11130	2329457..2329762	+	306	WP_012389218.1	HigA family addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2252101..2337045	84944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15348.94 Da
• Isoelectric Point: 9.7069

>T22827 WP_012389212.1 NC_010602:2324566-2324964 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
MYLIDTNICIYLIKKKNIKLLQKFQKNYSKGIFISSLTLAELEFGVENSEHKEKNRISLIEFLTIFEVLSFEQKDTFAFG
KIKSDLRKSGKMIGTMDALLAAQSVSRNLIFVTNNTKEFERINNLKIEDWTI

Nucleotide

• Length: 399 bp

>T22827 NC_010602:2324566-2324964 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
ATGTATCTAATTGATACAAATATTTGCATTTACCTTATTAAAAAGAAAAATATTAAACTCTTACAGAAATTTCAAAAGAA
TTATAGTAAAGGCATTTTTATTTCTTCACTCACTTTAGCTGAACTAGAATTTGGGGTAGAAAATAGTGAACATAAAGAGA
AAAATAGAATTTCTTTAATTGAATTTCTTACTATTTTTGAAGTTTTAAGCTTCGAGCAAAAAGACACTTTTGCATTCGGG
AAAATTAAAAGCGATTTAAGAAAATCAGGAAAAATGATTGGAACTATGGATGCATTATTAGCAGCCCAATCAGTTTCACG
AAACCTTATTTTTGTTACAAATAACACAAAAGAATTCGAAAGAATTAACAACTTGAAAATTGAGGATTGGACTATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8690.84 Da
• Isoelectric Point: 5.0644

>AT22827 WP_012389211.1 NC_010602:2324336-2324566 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
MQTAKIFVNGRSQAVRIPKEFQFKGEEVFIHKVGEAVVLVPKNKAWNTFLDGLNGFTDDFLSDGRIDLTESEREQF

Nucleotide

• Length: 231 bp

>AT22827 NC_010602:2324336-2324566 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
ATGCAAACAGCAAAAATTTTTGTCAATGGTAGAAGCCAAGCAGTCAGAATTCCAAAAGAATTTCAATTTAAAGGTGAGGA
AGTTTTTATACATAAAGTTGGAGAAGCTGTTGTTCTTGTTCCAAAAAATAAAGCATGGAATACGTTTTTAGATGGATTAA
ATGGTTTTACAGATGATTTTCTAAGTGACGGAAGGATTGATTTAACGGAATCAGAAAGAGAACAATTTTAA

Structures

Toxin

• AlphaFold DB: B0STA6

Antitoxin

• AlphaFold DB: B0STA5