Detailed information of TA system TA228265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2531850..2533438
• Replicon: chromosome
• Accession: NZ_CP089441
• Organism: Klebsiella quasipneumoniae strain GLW9C22

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LT990_RS12370
• Protein ID: WP_049014126.1
• Coordinates: 2531850..2533172 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: LT990_RS12375
• Protein ID: WP_023289667.1
• Coordinates: 2533172..2533438 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT990_RS12350 (2528785)	2528785..2529033	+	249	WP_004205912.1	hypothetical protein	-
LT990_RS12355 (2529325)	2529325..2529543	+	219	WP_002904808.1	ParD-like family protein	-
LT990_RS12360 (2529524)	2529524..2530306	+	783	WP_048297502.1	type I methionyl aminopeptidase	-
LT990_RS12365 (2530541)	2530541..2531833	+	1293	WP_044523014.1	glycoside hydrolase family 10 protein	-
LT990_RS12370 (2531850)	2531850..2533172	-	1323	WP_049014126.1	type II toxin-antitoxin system HipA family toxin	Toxin
LT990_RS12375 (2533172)	2533172..2533438	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LT990_RS12380 (2533703)	2533703..2534029	-	327	WP_044523006.1	hydroxyisourate hydrolase	-
LT990_RS12385 (2534026)	2534026..2534526	-	501	WP_217219846.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LT990_RS12390 (2534504)	2534504..2535883	-	1380	WP_217219845.1	nucleobase:cation symporter-2 family protein	-
LT990_RS12395 (2536229)	2536229..2537383	+	1155	WP_087827596.1	FAD-dependent urate hydroxylase HpxO	-
LT990_RS12400 (2537364)	2537364..2538287	-	924	WP_023289662.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49211.54 Da
• Isoelectric Point: 8.6110

>T228265 WP_049014126.1 NZ_CP089441:c2533172-2531850 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSVHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T228265 NZ_CP123598:1817283-1817567 [Enterobacter cloacae]
ATGACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAAGAGTGGAGCAAGCTGGGGGAGACTGTAAAAAAGCAGTTCAG
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCGGCCCGTCTGCATACCCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGGTAGTTGTCATCGCCATTGGGAAA
AGAGAGAAATCAGCCGTATATCACGATGCTAACAAACGGCTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT228265 WP_023289667.1 NZ_CP089441:c2533438-2533172 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT228265 NZ_CP123598:1817051-1817293 [Enterobacter cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTTTTTCAGTACGTTGCGGAGACGGGGCGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3