Detailed information of TA system TA228251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13609..14266
• Replicon: plasmid pHN21SC92-1
• Accession: NZ_CP089438
• Organism: Citrobacter portucalensis strain GD21SC92T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: LT980_RS25040
• Protein ID: WP_000270043.1
• Coordinates: 13916..14266 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LT980_RS25035
• Protein ID: WP_000124640.1
• Coordinates: 13609..13911 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT980_RS24985 (LT980_24975)	9234..9662	+	429	WP_000591074.1	hypothetical protein	-
LT980_RS24990 (LT980_24980)	9719..10078	+	360	WP_000422768.1	hypothetical protein	-
LT980_RS24995 (LT980_24985)	10078..10524	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
LT980_RS25000 (LT980_24990)	10521..11039	+	519	WP_000210756.1	nitrite reductase	-
LT980_RS25005 (LT980_24995)	11039..11269	+	231	WP_000972663.1	hypothetical protein	-
LT980_RS25010 (LT980_25000)	11256..12113	+	858	WP_001167032.1	hypothetical protein	-
LT980_RS25015 (LT980_25005)	12139..12327	+	189	WP_001270411.1	hypothetical protein	-
LT980_RS25020 (LT980_25010)	12344..12871	+	528	WP_001236377.1	thermonuclease family protein	-
LT980_RS25025 (LT980_25015)	12929..13201	+	273	WP_001043047.1	HU family DNA-binding protein	-
LT980_RS25030 (LT980_25020)	13289..13582	+	294	WP_001239997.1	chromosome segregation protein ParM	-
LT980_RS25035 (LT980_25025)	13609..13911	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
LT980_RS25040 (LT980_25030)	13916..14266	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LT980_RS25045 (LT980_25035)	14429..14977	+	549	WP_001061574.1	transcriptional regulator	-
LT980_RS25050 (LT980_25040)	15318..15512	+	195	WP_000343597.1	hypothetical protein	-
LT980_RS25055 (LT980_25045)	15523..15894	+	372	WP_000516916.1	hypothetical protein	-
LT980_RS25060 (LT980_25050)	15887..16357	+	471	WP_001281821.1	hypothetical protein	-
LT980_RS25065 (LT980_25055)	16372..16707	-	336	WP_000683476.1	hypothetical protein	-
LT980_RS25070 (LT980_25060)	16804..17292	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
LT980_RS25075 (LT980_25065)	17295..17792	+	498	WP_000062185.1	hypothetical protein	-
LT980_RS25080 (LT980_25070)	18007..18711	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LT980_RS25085 (LT980_25075)	18775..19113	+	339	Protein_32	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB / qepA1 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4	-	1..131627	131627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T228251 WP_000270043.1 NZ_CP089438:c14266-13916 [Citrobacter portucalensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T228251 NZ_CP123597:c2280843-2280445 [Serratia marcescens]
ATGTTCAGCCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCGTTCAA
TCGCTATGCGGGCAAAATGGTCATTTCGTCGGTCACCTATGGCGAATTGGTTCACGGAGTGGAGAAAAGCTCGCGTCCGG
CGGTTAACGCCAGAGTGGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACGGCGCCAAAGCGGCTTCGCACTAC
GGCAACATTCGGGCTGCGCTGGAACGTCAGGGAACGCCTATTGGCGTAAACGATCTGCATATCGCCGGGCATGCGCGCAG
CGAAGGATTGATTCTGGTGACCAATAACCGACGCGAATTTGAGCGCGTTGATGGCTTACGGCTGGAAAATTGGCTGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT228251 WP_000124640.1 NZ_CP089438:c13911-13609 [Citrobacter portucalensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT228251 NZ_CP123597:c2281076-2280843 [Serratia marcescens]
ATGACGTTAGGTTCTGTCTTTACCAATAATCGGACGCAGGCCGTGCGCTTGCCTGCAGAGGTGCGTTTCCCCGATACAGT
TTCTAAAGTGACCGTCCGCGTAGTGGGCAAAGAGCGCATTCTTGCGCCGGTGGAGAATACCTGGGACAGTTTCTTCTGCG
CGGAAAATGAGGTGAGCGATGACTTCCTCAATGAACGTGCCGGGCAACACCAGCGCGAGCGGGAAGAACTTTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9