Detailed information of TA system TA228250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4952766..4953520
• Replicon: chromosome
• Accession: NZ_CP089437
• Organism: Citrobacter portucalensis strain GD21SC92T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LT980_RS23990
• Protein ID: WP_250671535.1
• Coordinates: 4952766..4953077 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LT980_RS23995
• Protein ID: WP_048215203.1
• Coordinates: 4953074..4953520 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT980_RS23960 (LT980_23950)	4948433..4949335	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
LT980_RS23965 (LT980_23955)	4949332..4949967	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
LT980_RS23970 (LT980_23960)	4949964..4950893	+	930	WP_237857414.1	formate dehydrogenase accessory protein FdhE	-
LT980_RS23975 (LT980_23965)	4950930..4951304	-	375	WP_020996118.1	type II toxin-antitoxin system VapC family toxin	-
LT980_RS23980 (LT980_23970)	4951304..4951546	-	243	WP_020996119.1	CopG family transcriptional regulator	-
LT980_RS23985 (LT980_23975)	4951752..4952681	+	930	WP_250671534.1	alpha/beta hydrolase	-
LT980_RS23990 (LT980_23980)	4952766..4953077	+	312	WP_250671535.1	type II toxin-antitoxin system HigB family toxin	Toxin
LT980_RS23995 (LT980_23985)	4953074..4953520	+	447	WP_048215203.1	helix-turn-helix domain-containing protein	Antitoxin
LT980_RS24000 (LT980_23990)	4953538..4954479	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
LT980_RS24005 (LT980_23995)	4954524..4954961	-	438	WP_003825289.1	D-aminoacyl-tRNA deacylase	-
LT980_RS24010 (LT980_24000)	4954958..4955830	-	873	WP_008786998.1	virulence factor BrkB family protein	-
LT980_RS24015 (LT980_24005)	4955824..4956423	-	600	WP_008786997.1	glucose-1-phosphatase	-
LT980_RS24020 (LT980_24010)	4956514..4957404	-	891	WP_111466284.1	aldose 1-epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12361.34 Da
• Isoelectric Point: 9.2624

>T228250 WP_250671535.1 NZ_CP089437:4952766-4953077 [Citrobacter portucalensis]
VHVISRKPFNEAMLMYPNHQLALADLLNVLEKKTFIQPEEMKQYIPSLDNFKYRDKWWVIDVSGNCLRLISYIDFRSHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T228250 NZ_CP123597:2238565-2238768 [Serratia marcescens]
GTGAAGAGTTCAGAACTGATTGCGCTGCTGGAAAGCCACGGTTGGGTATTGCGGCGGGTAAAAGGCAGCCACCATCAATT
CAAACATCCCGATTTTGCGCTGATCATCACCGTGCCGCATCCAAAGAAAGATCTCAAGCTGGGCACGCTGCGGCAGATAC
TTAAAGACGCGGGGTTAAGTGCGCTTCACATCGCCACGCGTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16660.97 Da
• Isoelectric Point: 6.7580

>AT228250 WP_048215203.1 NZ_CP089437:4953074-4953520 [Citrobacter portucalensis]
MRTHRHMDAASAKKIVDTFSDAVKTIPLLGEDRNDTEYRRALALVEYLVDHDDLENPLFELLCARISKYEKHAPEFKALN
QHLEKTPSGVSVLRTLMDQYSLKAADLANELGSKSNVSNILNGRRALTVSHIKALAERFNLPADAFIE

Nucleotide

• Length: 447 bp

>AT228250 NZ_CP123597:2238780-2239193 [Serratia marcescens]
ATGTTTTATCCCGCTTATATCCATACAGAATCAAACGGCAGCGCCAGCGGTTTCTTTCCCGACGTCCCTGGCTGCTACTT
CGCCGGCACCAGCCTGGACGACGCTTTTGCCGATGCCCAAAGCGCACTGGATGCGCACTTTGAGTTGTTGAGCGAAGACA
ATCAACCGCTACCGCGCCCGCATGCCGTCGCAGCCCATCTTGCCAAAGATGCGGGATCCTTCGAGGGAGGACAATGGCTA
TTGGTCGCTATCAATATGGATAAATTTGACGGCCGAGCCGAACGGATTAACATCACGTTGCCCCATCGCCTGTTAAGTCG
CATTGACTCTCTGGTTCGGCAACATCCGGGATACGGCAGCCGCAGCGGATTTCTCGCCGCCGCCGCGCGCAATGAATTGT
TGAAAGCAGAGTGA