Detailed information of TA system TA022825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 757029..757594
• Replicon: chromosome
• Accession: NC_010602
• Organism: Leptospira biflexa serovar Patoc strain Patoc 1 (Paris)

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B0SL65
• Locus tag: LEPBI_RS03650
• Protein ID: WP_012387759.1
• Coordinates: 757029..757277 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B0SL66
• Locus tag: LEPBI_RS03655
• Protein ID: WP_012387760.1
• Coordinates: 757271..757594 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEPBI_RS03640	752584..755145	+	2562	WP_012387757.1	adenylate/guanylate cyclase domain-containing protein	-
LEPBI_RS03645	755184..756884	-	1701	WP_012387758.1	flagellar M-ring protein FliF	-
LEPBI_RS03650	757029..757277	+	249	WP_012387759.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
LEPBI_RS03655	757271..757594	+	324	WP_012387760.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
LEPBI_RS03660	757654..759120	-	1467	WP_012387761.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-
LEPBI_RS03665	759288..759944	+	657	WP_012387762.1	TetR/AcrR family transcriptional regulator	-
LEPBI_RS03670	759941..761146	-	1206	WP_012387763.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (7-48)

Antitoxin

• mRNA interferase PemK-like (4-105)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9382.69 Da
• Isoelectric Point: 4.2220

>T22825 WP_012387759.1 NC_010602:757029-757277 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
MKVAVVQIGNSKGIRIPKTVLAECQIEDAVDLQIEGDKIIISPYKQRPRQGWSTQFQEMAKENDDSLLLPDVLEQEVEGW
EW

Nucleotide

• Length: 249 bp

>T22825 NC_010602:757029-757277 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
ATGAAAGTAGCAGTCGTGCAGATTGGTAATTCCAAAGGCATTCGAATCCCCAAGACAGTTCTTGCAGAATGCCAGATCGA
AGATGCAGTGGATTTGCAGATAGAGGGTGATAAAATTATCATCTCTCCGTATAAACAGAGACCAAGACAAGGTTGGTCGA
CCCAATTCCAAGAGATGGCAAAAGAGAATGATGATTCACTCCTTTTGCCAGATGTTTTGGAACAAGAGGTTGAGGGCTGG
GAATGGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12316.48 Da
• Isoelectric Point: 9.8627

>AT22825 WP_012387760.1 NC_010602:757271-757594 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
MVTKQYDIYLVNLDPTVGHEIQKSRPCIVISPNEMNEFIRTVMIAPMTTVMREYPTRVSISFQGKKGFIVLDQIRTVDQS
RLMQKLGSSDPKTNQKIKNVIKEMLVD

Nucleotide

• Length: 324 bp

>AT22825 NC_010602:757271-757594 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
ATGGTGACGAAACAATATGATATCTACCTCGTCAATTTGGATCCAACTGTTGGACATGAAATCCAAAAATCAAGACCTTG
CATTGTGATCTCTCCAAATGAGATGAACGAATTCATACGCACTGTGATGATCGCTCCTATGACAACTGTAATGAGAGAAT
ACCCAACTCGGGTATCCATTTCGTTTCAAGGTAAAAAAGGTTTCATAGTATTGGACCAGATACGAACTGTGGACCAATCG
AGATTGATGCAAAAACTTGGTTCTTCTGATCCGAAAACGAATCAAAAAATTAAGAATGTGATCAAAGAAATGTTAGTTGA
TTGA

Structures

Toxin

• AlphaFold DB: B0SL65

Antitoxin

• AlphaFold DB: B0SL66