Detailed information of TA system TA228247

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 4652919..4653712
• Replicon: chromosome
• Accession: NZ_CP089437
• Organism: Citrobacter portucalensis strain GD21SC92T

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: LT980_RS22615
• Protein ID: WP_000854726.1
• Coordinates: 4653335..4653712 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: LT980_RS22610
• Protein ID: WP_001548930.1
• Coordinates: 4652919..4653245 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT980_RS22565 (LT980_22555)	4648359..4649180	+	822	WP_000197393.1	DUF932 domain-containing protein	-
LT980_RS22570 (LT980_22560)	4649398..4650099	+	702	WP_001548933.1	WYL domain-containing protein	-
LT980_RS22575 (LT980_22565)	4650136..4650372	+	237	WP_001115854.1	protein YpjK	-
LT980_RS22580 (LT980_22570)	4650372..4650815	+	444	WP_000824224.1	hypothetical protein	-
LT980_RS22585 (LT980_22575)	4650839..4651306	+	468	WP_115799765.1	protein YkfB	-
LT980_RS22590 (LT980_22580)	4651383..4651622	+	240	WP_000194654.1	DUF905 family protein	-
LT980_RS22595 (LT980_22585)	4651720..4652178	+	459	WP_115799766.1	antirestriction protein	-
LT980_RS22600 (LT980_22590)	4652194..4652670	+	477	WP_000811693.1	RadC family protein	-
LT980_RS22605 (LT980_22595)	4652679..4652900	+	222	WP_000691994.1	DUF987 domain-containing protein	-
LT980_RS22610 (LT980_22600)	4652919..4653245	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LT980_RS22615 (LT980_22605)	4653335..4653712	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
LT980_RS22620 (LT980_22610)	4653709..4654197	+	489	WP_115799767.1	DUF5983 family protein	-
LT980_RS22625 (LT980_22615)	4654209..4654406	+	198	WP_115799768.1	DUF957 domain-containing protein	-
LT980_RS22630 (LT980_22620)	4654491..4655333	+	843	WP_001548928.1	DUF4942 domain-containing protein	-
LT980_RS22635 (LT980_22625)	4655549..4655866	-	318	WP_250671487.1	hypothetical protein	-
LT980_RS22640 (LT980_22630)	4655916..4657577	+	1662	WP_250671488.1	fatty acid--CoA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T228247 WP_000854726.1 NZ_CP089437:4653335-4653712 [Citrobacter portucalensis]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T228247 NZ_CP123597:c229311-229108 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCAGATCATCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT228247 WP_001548930.1 NZ_CP089437:4652919-4653245 [Citrobacter portucalensis]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT228247 NZ_CP123597:c229729-229361 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7