Detailed information of TA system TA228198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 108693..109215
• Replicon: plasmid pKOX101_1
• Accession: NZ_CP089408
• Organism: Klebsiella michiganensis strain Kox101

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: LUW94_RS29595
• Protein ID: WP_004187019.1
• Coordinates: 108693..108977 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: LUW94_RS29600
• Protein ID: WP_004187025.1
• Coordinates: 108967..109215 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW94_RS29570 (LUW94_29570)	103768..104691	-	924	WP_023328909.1	cation diffusion facilitator family transporter	-
LUW94_RS29575 (LUW94_29575)	104952..105272	+	321	WP_049084165.1	metalloregulator ArsR/SmtB family transcription factor	-
LUW94_RS29580 (LUW94_29580)	105318..106607	+	1290	WP_035897525.1	arsenite efflux transporter membrane subunit ArsB	-
LUW94_RS29585 (LUW94_29585)	106620..107048	+	429	WP_035897523.1	glutaredoxin-dependent arsenate reductase	-
LUW94_RS29590 (LUW94_29590)	107334..108677	+	1344	WP_049084170.1	ISNCY family transposase	-
LUW94_RS29595 (LUW94_29595)	108693..108977	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LUW94_RS29600 (LUW94_29600)	108967..109215	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
LUW94_RS29605 (LUW94_29605)	109593..109991	-	399	WP_049084174.1	helix-turn-helix domain-containing protein	-
LUW94_RS29610 (LUW94_29610)	110040..111386	-	1347	WP_077259693.1	ISNCY family transposase	-
LUW94_RS29615 (LUW94_29615)	111515..112285	+	771	WP_182205113.1	hypothetical protein	-
LUW94_RS29625 (LUW94_29625)	113031..113345	-	315	WP_074181342.1	hypothetical protein	-
LUW94_RS29630 (LUW94_29630)	113372..113773	-	402	WP_177343058.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..220391	220391
	-	inside	IScluster/Tn	-	-	110040..125000	14960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T228198 WP_004187019.1 NZ_CP089408:c108977-108693 [Klebsiella michiganensis]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T228198 NZ_CP123498:1458541-1458858 [Arsenophonus nasoniae]
ATGAAAGAACGTAAACTAAAACAAACCGCCGTTTTTCATGCCTGGGAATCCAAGTTAAGGGATCGTCGGGCGAGAACCTT
GATTGCCACGCGTTTGTTTCGCCTGGCGAATGGATTAGCGGGCGATGTTATGCCGGTCGGGGAAGGGATCAGCGAACTGC
GTATTCATTATGGCCCGGGCTATCGGATTTATTTTCAACAGCGTGGTGACACGATTATTGTTTTGTTATGCGGTGGTGAT
AAAAGCACACAGTCCAACGATATTTTATTGGCTAAGAAATTAGCGAAAGAGTGGATTGATGAGGTGAATGATGATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT228198 WP_004187025.1 NZ_CP089408:c109215-108967 [Klebsiella michiganensis]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT228198 NZ_CP123498:1458848-1459135 [Arsenophonus nasoniae]
ATGATGATTAAAAAATTAACAACTTATGATCCGGCTGAGGCGTTGGTCAATGATGAAGAAATTGCCTTTTTTATGGCCGA
TGCGCTGGAAACAGGCGATGCAAGTTATATCGCTAAAGCTATCGGTGTAGTGGCTCGGGCAAAGGGCATGACACAAATAG
CGGCACAAACCGGGCTATCCCGTGAACAGCTCTATCGCTCATTTAGTGACGAAGGTAACCCAACCTTAAAAACCACTTTG
GCAGTAATGAAAGCATTAGGCGTTGATTTAACCGTTAAACGCCATTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4