Detailed information of TA system TA228193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4012695..4013271
• Replicon: chromosome
• Accession: NZ_CP089407
• Organism: Klebsiella michiganensis strain Kox101

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1D8JNH2
• Locus tag: LUW94_RS19045
• Protein ID: WP_042933975.1
• Coordinates: 4012984..4013271 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A1D8JNF7
• Locus tag: LUW94_RS19040
• Protein ID: WP_025108476.1
• Coordinates: 4012695..4012997 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW94_RS19020 (LUW94_19020)	4007885..4009180	+	1296	WP_143440150.1	restriction endonuclease subunit S	-
LUW94_RS19025 (LUW94_19025)	4009531..4009866	+	336	WP_046878037.1	endoribonuclease SymE	-
LUW94_RS19030 (LUW94_19030)	4010323..4011234	+	912	WP_202019351.1	acetamidase/formamidase family protein	-
LUW94_RS19035 (LUW94_19035)	4011231..4012574	+	1344	WP_004128506.1	APC family permease	-
LUW94_RS19040 (LUW94_19040)	4012695..4012997	-	303	WP_025108476.1	BrnA antitoxin family protein	Antitoxin
LUW94_RS19045 (LUW94_19045)	4012984..4013271	-	288	WP_042933975.1	BrnT family toxin	Toxin
LUW94_RS19050 (LUW94_19050)	4013523..4013966	-	444	WP_202019352.1	FosA family fosfomycin resistance glutathione transferase	-
LUW94_RS19055 (LUW94_19055)	4013960..4014868	-	909	WP_049080873.1	LysR family transcriptional regulator	-
LUW94_RS19060 (LUW94_19060)	4014956..4015738	+	783	WP_202019353.1	NAD(P)H-dependent oxidoreductase	-
LUW94_RS19065 (LUW94_19065)	4015886..4016470	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
LUW94_RS19070 (LUW94_19070)	4016616..4017416	+	801	WP_046878031.1	winged helix-turn-helix domain-containing protein	-
LUW94_RS19075 (LUW94_19075)	4017413..4017931	+	519	WP_004098240.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T228193 WP_042933975.1 NZ_CP089407:c4013271-4012984 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T228193 NZ_CP123498:c545396-545100 [Arsenophonus nasoniae]
ATGATTGAAATCAGGCAAACCGAAGAAGTTAAAAAATGGCTAAAAGGATTAAAAGATAAAATAGCCAAAGCAAAAATTTT
AACACGGATTGAACGGATGAAAGAAGGTAACTATGGGGATGTAGAACCGATAGGTGATGGATATTCAGAATTAAAAATCC
ATCAAGGCAAAGGCTACAGGGTATATTTTGCCACACGGAACAATGAAATTATTTTATTGCTTTGTGGTGGAGATAAAGCA
ACACAACAAGCCGATATTAAAAAAGCAAAGCAGATAGCGAAACAATGGGGGTTCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11563.17 Da
• Isoelectric Point: 9.7962

>AT228193 WP_025108476.1 NZ_CP089407:c4012997-4012695 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT228193 NZ_CP123498:c545098-544814 [Arsenophonus nasoniae]
ATGAAAACAAAACCAGAATTATTTGATGTGGTCGATTACCTCGATAATGAAGAAGATATACAGGCATATCTAAATGCTGC
GCTGGATGAAGACGATGAAAACTATTTTTTAGTGGCACTAGGAAACGTTGCCAGAGCAAGAAATATTAGTCACCTTTCAA
AAGAAACTGGAATGAGCCGTGAAGGAATTTACAAGGCACTTTCCGGTGCGGGAAATCCATCATTTAAAACTATATCAAAA
ATCTCTAAAGCATTAGGTTTAAAATTACACTTTTCAGGGGCCTAA

Structures

Toxin

• AlphaFold DB: A0A1D8JNH2

Antitoxin

• AlphaFold DB: A0A1D8JNF7