Detailed information of TA system TA227964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3560211..3560689
• Replicon: chromosome
• Accession: NZ_CP089272
• Organism: Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: LSI73_RS18540
• Protein ID: WP_001303876.1
• Coordinates: 3560402..3560689 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: LSI73_RS18535
• Protein ID: WP_000536233.1
• Coordinates: 3560211..3560402 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI73_RS18500 (3555609)	3555609..3556379	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
LSI73_RS18505 (3556401)	3556401..3557147	-	747	WP_000788745.1	ATP-binding protein	-
LSI73_RS18510 (3557154)	3557154..3558251	-	1098	WP_001475117.1	hypothetical protein	-
LSI73_RS18515 (3558330)	3558330..3558785	+	456	WP_000273724.1	hypothetical protein	-
LSI73_RS18520 (3558992)	3558992..3559417	-	426	WP_000693855.1	toxin YdaT family protein	-
LSI73_RS18525 (3559401)	3559401..3559673	-	273	WP_000887453.1	YdaS family helix-turn-helix protein	-
LSI73_RS18530 (3559782)	3559782..3560183	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
LSI73_RS18535 (3560211)	3560211..3560402	+	192	WP_000536233.1	hypothetical protein	Antitoxin
LSI73_RS18540 (3560402)	3560402..3560689	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSI73_RS18545 (3560967)	3560967..3561122	+	156	WP_000379575.1	DUF1391 family protein	-
LSI73_RS28225 (3561124)	3561124..3561252	+	129	WP_000344963.1	protein YdfB	-
LSI73_RS18550 (3561264)	3561264..3561653	+	390	WP_000394511.1	hypothetical protein	-
LSI73_RS18555 (3561840)	3561840..3562025	-	186	WP_001133046.1	hypothetical protein	-
LSI73_RS18560 (3562599)	3562599..3562787	+	189	WP_000413705.1	cell division inhibition protein DicB	-
LSI73_RS18565 (3562784)	3562784..3562975	+	192	WP_001098307.1	DUF1482 family protein	-
LSI73_RS18570 (3563069)	3563069..3565540	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T227964 WP_001303876.1 NZ_CP089272:3560402-3560689 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T227964 NZ_CP123240:c4364437-4364150 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT227964 WP_000536233.1 NZ_CP089272:3560211-3560402 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT227964 NZ_CP123240:c4364163-4363861 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA