Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2839249..2839887 Replicon chromosome
Accession NZ_CP089272
Organism Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LSI73_RS14565 Protein ID WP_000813794.1
Coordinates 2839711..2839887 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LSI73_RS14560 Protein ID WP_001270286.1
Coordinates 2839249..2839665 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI73_RS14540 (2834401) 2834401..2835342 - 942 WP_001251315.1 ABC transporter permease -
LSI73_RS14545 (2835343) 2835343..2836356 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
LSI73_RS14550 (2836374) 2836374..2837519 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
LSI73_RS14555 (2837764) 2837764..2839170 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
LSI73_RS14560 (2839249) 2839249..2839665 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LSI73_RS14565 (2839711) 2839711..2839887 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LSI73_RS14570 (2840109) 2840109..2840339 + 231 WP_000494244.1 YncJ family protein -
LSI73_RS14575 (2840431) 2840431..2842392 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LSI73_RS14580 (2842465) 2842465..2843001 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
LSI73_RS14585 (2843093) 2843093..2844266 + 1174 Protein_2854 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T227960 WP_000813794.1 NZ_CP089272:c2839887-2839711 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T227960 NZ_CP123240:3297179-3297565 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT227960 WP_001270286.1 NZ_CP089272:c2839665-2839249 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT227960 NZ_CP123240:3297555-3297884 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References