Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3380516..3381311 | Replicon | chromosome |
| Accession | NZ_CP089270 | ||
| Organism | Escherichia coli O157:H7 strain M1300706002 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | B7UP43 |
| Locus tag | LSI72_RS17505 | Protein ID | WP_000854914.1 |
| Coordinates | 3380516..3380890 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0B0VS41 |
| Locus tag | LSI72_RS17510 | Protein ID | WP_001280954.1 |
| Coordinates | 3380937..3381311 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LSI72_RS17465 (3375520) | 3375520..3376011 | - | 492 | WP_001301587.1 | DUF1097 domain-containing protein | - |
| LSI72_RS17470 (3376113) | 3376113..3376667 | - | 555 | WP_001001917.1 | molecular chaperone YcdY | - |
| LSI72_RS17475 (3376691) | 3376691..3377428 | - | 738 | WP_000283664.1 | zinc-binding phosphatase | - |
| LSI72_RS17480 (3377483) | 3377483..3378421 | - | 939 | WP_000351284.1 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| LSI72_RS17490 (3378892) | 3378892..3379734 | - | 843 | WP_001280481.1 | DUF4942 domain-containing protein | - |
| LSI72_RS17495 (3379819) | 3379819..3380016 | - | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| LSI72_RS17500 (3380028) | 3380028..3380519 | - | 492 | WP_000976853.1 | DUF5983 family protein | - |
| LSI72_RS17505 (3380516) | 3380516..3380890 | - | 375 | WP_000854914.1 | TA system toxin CbtA family protein | Toxin |
| LSI72_RS17510 (3380937) | 3380937..3381311 | - | 375 | WP_001280954.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| LSI72_RS17515 (3381474) | 3381474..3381695 | - | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| LSI72_RS17520 (3381758) | 3381758..3382234 | - | 477 | WP_001186738.1 | RadC family protein | - |
| LSI72_RS17525 (3382250) | 3382250..3382735 | - | 486 | WP_000214398.1 | antirestriction protein | - |
| LSI72_RS17530 (3382826) | 3382826..3383644 | - | 819 | WP_001234682.1 | DUF932 domain-containing protein | - |
| LSI72_RS17535 (3383984) | 3383984..3385054 | - | 1071 | WP_000102669.1 | patatin-like phospholipase family protein | - |
| LSI72_RS17540 (3385051) | 3385051..3385956 | - | 906 | WP_000203541.1 | diguanylate cyclase regulator RdcB family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | csgB / csgD / csgE / csgF / csgG | 3371902..3382735 | 10833 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14117.17 Da Isoelectric Point: 7.7761
>T227928 WP_000854914.1 NZ_CP089270:c3380890-3380516 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T227928 NZ_CP123237:958715-959050 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTCG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTCG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
Antitoxin
Download Length: 125 a.a. Molecular weight: 13718.51 Da Isoelectric Point: 6.6249
>AT227928 WP_001280954.1 NZ_CP089270:c3381311-3380937 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT227928 NZ_CP123237:958467-958715 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LXR5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0B0VS41 |