Detailed information of TA system TA022768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 1015606..1016200
• Replicon: chromosome
• Accession: NC_010519
• Organism: Haemophilus somnus 2336

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B0USW1
• Locus tag: HSM_RS04550
• Protein ID: WP_012341688.1
• Coordinates: 1015606..1015902 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HSM_RS04555
• Protein ID: WP_012341689.1
• Coordinates: 1015904..1016200 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HSM_RS04515	1010620..1011249	-	630	WP_012341682.1	hypothetical protein	-
HSM_RS04520	1011246..1011908	-	663	WP_012341683.1	AAA family ATPase	-
HSM_RS10455	1011972..1012604	-	633	WP_012341684.1	KilA-N domain-containing protein	-
HSM_RS04530	1012882..1013709	-	828	WP_012341685.1	hypothetical protein	-
HSM_RS04535	1014064..1014279	+	216	WP_041605053.1	hypothetical protein	-
HSM_RS04540	1014768..1014989	-	222	WP_012341686.1	hypothetical protein	-
HSM_RS04545	1015001..1015189	+	189	WP_012341687.1	DUF1508 domain-containing protein	-
HSM_RS04550	1015606..1015902	+	297	WP_012341688.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HSM_RS04555	1015904..1016200	+	297	WP_012341689.1	putative addiction module antidote protein	Antitoxin
HSM_RS04560	1016228..1017232	-	1005	WP_012341691.1	hypothetical protein	-
HSM_RS04565	1017329..1018015	-	687	WP_012341692.1	XRE family transcriptional regulator	-
HSM_RS04570	1018118..1018345	+	228	WP_012341693.1	hypothetical protein	-
HSM_RS04575	1018498..1018737	+	240	WP_012341694.1	helix-turn-helix domain-containing protein	-
HSM_RS04580	1018727..1019551	+	825	WP_012341695.1	hypothetical protein	-
HSM_RS04585	1019548..1020240	+	693	WP_011609555.1	replication protein P	-
HSM_RS04590	1020255..1020788	+	534	WP_012341696.1	phage N-6-adenine-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	999788..1054251	54463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11127.10 Da
• Isoelectric Point: 10.5997

>T22768 WP_012341688.1 NC_010519:1015606-1015902 [Histophilus somni 2336]
MIQIKSTTIFKKWLDDLKDLRARAKIQTRIKRLQLGNFGDVKTVGEGIFELRITEGKGYRVYFKNQNGVIVILLCAGDKS
TQENDIKKAKTLAKELGV

Nucleotide

• Length: 297 bp

>T22768 NC_010519:1015606-1015902 [Histophilus somni 2336]
ATGATCCAAATAAAATCAACCACAATTTTTAAGAAATGGCTTGATGATTTAAAGGATTTGAGAGCAAGGGCAAAGATCCA
AACAAGAATTAAACGCTTACAGCTGGGTAACTTTGGTGATGTAAAAACTGTAGGAGAAGGAATATTCGAATTACGCATTA
CTGAGGGCAAAGGCTATCGTGTTTATTTCAAAAATCAGAATGGTGTGATTGTAATTTTACTTTGTGCTGGTGATAAATCT
ACACAGGAAAATGATATTAAAAAAGCAAAAACTTTAGCAAAAGAATTGGGGGTATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10892.51 Da
• Isoelectric Point: 4.8605

>AT22768 WP_012341689.1 NC_010519:1015904-1016200 [Histophilus somni 2336]
MTELQDFDIAEHLRNEEEIRFYLNEVMKEGDVDLILSALGDIAKARNMSQLAKEVGVSREGLYKAFSGKGNPTFSTVLKV
MKALNLSLEIKQSAVSLS

Nucleotide

• Length: 297 bp

>AT22768 NC_010519:1015904-1016200 [Histophilus somni 2336]
ATGACTGAATTACAAGATTTTGATATAGCGGAGCATTTAAGAAATGAGGAAGAAATCCGTTTTTATCTAAATGAAGTGAT
GAAAGAGGGGGATGTAGATTTAATTCTTTCCGCACTAGGTGATATTGCCAAAGCACGTAATATGAGCCAATTGGCAAAAG
AGGTCGGGGTAAGTCGTGAGGGATTATATAAAGCCTTTTCCGGCAAAGGTAACCCGACATTTTCTACTGTGCTTAAGGTA
ATGAAAGCATTGAATTTAAGCCTCGAGATTAAACAGTCTGCCGTCAGTTTGAGTTAA

Structures

Toxin

• AlphaFold DB: B0USW1