Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 911599..912253 Replicon chromosome
Accession NZ_CP089142
Organism Escherichia coli strain XD35

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K0768_RS07815 Protein ID WP_000244777.1
Coordinates 911846..912253 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K0768_RS07810 Protein ID WP_000354046.1
Coordinates 911599..911865 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0768_RS07785 (906768) 906768..907511 + 744 WP_000951964.1 SDR family oxidoreductase -
K0768_RS07790 (907568) 907568..909001 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
K0768_RS07795 (909046) 909046..909357 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
K0768_RS07800 (909521) 909521..910180 + 660 WP_000250272.1 hemolysin III family protein -
K0768_RS07805 (910376) 910376..911356 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K0768_RS07810 (911599) 911599..911865 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K0768_RS07815 (911846) 911846..912253 + 408 WP_000244777.1 protein YgfX Toxin
K0768_RS07820 (912293) 912293..912814 - 522 WP_001055874.1 flavodoxin FldB -
K0768_RS07825 (912926) 912926..913822 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K0768_RS07830 (913847) 913847..914557 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K0768_RS07835 (914563) 914563..916296 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T227674 WP_000244777.1 NZ_CP089142:911846-912253 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T227674 NZ_CP123013:1111669-1111980 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT227674 WP_000354046.1 NZ_CP089142:911599-911865 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT227674 NZ_CP123013:1111977-1112396 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References