Detailed information of TA system TA227585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4286894..4287480
• Replicon: chromosome
• Accession: NZ_CP089063
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF37

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: LSP18_RS19960
• Protein ID: WP_003120987.1
• Coordinates: 4287181..4287480 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LSP18_RS19955
• Protein ID: WP_003448662.1
• Coordinates: 4286894..4287184 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP18_RS19935 (LSP18_20105)	4282045..4282254	+	210	WP_003105733.1	cold-shock protein	-
LSP18_RS19940 (LSP18_31800)	4282476..4284365	+	1890	WP_043502704.1	hypothetical protein	-
LSP18_RS19945 (LSP18_20120)	4284362..4286338	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
LSP18_RS19950 (LSP18_20125)	4286479..4286823	+	345	WP_016851612.1	hypothetical protein	-
LSP18_RS19955 (LSP18_20130)	4286894..4287184	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
LSP18_RS19960 (LSP18_20135)	4287181..4287480	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSP18_RS19965 (LSP18_20140)	4287682..4288806	+	1125	WP_034024019.1	TcpQ domain-containing protein	-
LSP18_RS19970 (LSP18_20150)	4288806..4290515	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
LSP18_RS19975 (LSP18_20155)	4290519..4291844	+	1326	WP_242924069.1	type 4b pilus protein PilO2	-
LSP18_RS19980 (LSP18_20160)	4291834..4292367	+	534	WP_023094340.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4259915..4356127	96212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T227585 WP_003120987.1 NZ_CP089063:c4287480-4287181 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T227585 NZ_CP122997:78921-79247 [Mycolicibacterium aubagnense]
GTGATTCGCGGAGAAATTTGGACAGCATCCGGCCGTGACTACGCCGGCAAACCGCGGCCAGTGCTCGTCGTCCAGGACGA
CCGCTTCGACGCCACGGATTCCGTCACGATCTGCCCGCTCACAACGAATGACGCCGGCGCACCTCTGTTACGAATACCCT
TGCAGCCCAACGCCTACAACGGACTCGTAACTTTGAGCCACATCATGGTCGACAAGATCACCACGGTTCCCAGGGCCAAG
ATCGGCCAACGCATCGGCAAGATATCAACCACTGAGATGCTGCAACTCGAACGCGGACTACTCGTCTTCCTCGGAATGGC
CGGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT227585 WP_003448662.1 NZ_CP089063:c4287184-4286894 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT227585 NZ_CP122997:78697-78924 [Mycolicibacterium aubagnense]
ATGGCCAACCGCGACATCAACAGGCGAGTCGCCGACCACCGCACCCGCCTGCGCGAACAAGGACTGCGCCCACTCCAAAT
ATGGGTACCCGACACTCGCGCACCAGGATTCGCAGAGGAAGCTCACCGCCAGTCAGCCCTCGTAGCCGCCTCCAGCAGCG
CAGCCGAGGACCAGGCCTTCGTGGACAATATCTCGCAGTTCAATGATGAGGACTTCGACGCCGAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73