Detailed information of TA system TA227549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4913485..4914080
• Replicon: chromosome
• Accession: NZ_CP089036
• Organism: Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: CO544_RS24675
• Protein ID: WP_000239579.1
• Coordinates: 4913485..4913835 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: CO544_RS24680
• Protein ID: WP_001223210.1
• Coordinates: 4913829..4914080 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO544_RS24655 (4908931)	4908931..4909953	-	1023	WP_001301928.1	ABC transporter permease	-
CO544_RS24660 (4909967)	4909967..4911469	-	1503	Protein_4842	sugar ABC transporter ATP-binding protein	-
CO544_RS24665 (4911609)	4911609..4912565	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CO544_RS24670 (4912875)	4912875..4913405	+	531	WP_000055075.1	inorganic diphosphatase	-
CO544_RS24675 (4913485)	4913485..4913835	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
CO544_RS24680 (4913829)	4913829..4914080	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
CO544_RS24685 (4914292)	4914292..4914633	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CO544_RS24690 (4914636)	4914636..4918415	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T227549 WP_000239579.1 NZ_CP089036:c4913835-4913485 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T227549 NZ_CP122938:c4598082-4597909 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT227549 WP_001223210.1 NZ_CP089036:c4914080-4913829 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT227549 NZ_CP122938:c4598406-4598110 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8