Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-HTH_XRE
Location 3714398..3715103 Replicon chromosome
Accession NZ_CP089036
Organism Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)


Gene name relE Uniprot ID Q8X3N0
Locus tag CO544_RS19210 Protein ID WP_000539519.1
Coordinates 3714398..3714784 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag CO544_RS19215 Protein ID WP_001280945.1
Coordinates 3714774..3715103 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO544_RS19190 (3710402) 3710402..3711028 + 627 WP_001295292.1 glutathione S-transferase GstB -
CO544_RS19195 (3711025) 3711025..3712140 - 1116 WP_000554959.1 aldose sugar dehydrogenase YliI -
CO544_RS19200 (3712251) 3712251..3712634 - 384 WP_000497137.1 biofilm formation regulator BssR -
CO544_RS19205 (3712847) 3712847..3714172 + 1326 WP_000049367.1 30S ribosomal protein S12 methylthiotransferase RimO -
CO544_RS19210 (3714398) 3714398..3714784 + 387 WP_000539519.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CO544_RS19215 (3714774) 3714774..3715103 + 330 WP_001280945.1 DNA-binding transcriptional regulator Antitoxin
CO544_RS19220 (3715173) 3715173..3716501 - 1329 WP_000086905.1 GGDEF domain-containing protein -
CO544_RS19225 (3716509) 3716509..3718857 - 2349 WP_000950320.1 EAL domain-containing protein -
CO544_RS19230 (3719035) 3719035..3719946 - 912 WP_001236044.1 glutathione ABC transporter permease GsiD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(1-124)

Antitoxin

Cro/C1-type helix-turn-helix domain

(53-94)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14279.42 Da        Isoelectric Point: 9.9296

>T227543 WP_000539519.1 NZ_CP089036:3714398-3714784 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Download         Length: 387 bp

>T227543 NZ_CP122938:3327613-3327999 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCACTAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11982.08 Da        Isoelectric Point: 10.0101

>AT227543 WP_001280945.1 NZ_CP089036:3714774-3715103 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Download         Length: 330 bp

>AT227543 NZ_CP122938:3327989-3328318 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References