Detailed information of TA system TA227523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 702436..703235
• Replicon: chromosome
• Accession: NZ_CP089036
• Organism: Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: CO544_RS03455
• Protein ID: WP_000347273.1
• Coordinates: 702436..702900 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: CO544_RS03460
• Protein ID: WP_001302819.1
• Coordinates: 702900..703235 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO544_RS03425 (697437)	697437..697871	-	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
CO544_RS03430 (697889)	697889..698767	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
CO544_RS03435 (698757)	698757..699536	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
CO544_RS03440 (699547)	699547..700020	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CO544_RS03445 (700043)	700043..701323	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CO544_RS03450 (701572)	701572..702381	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CO544_RS03455 (702436)	702436..702900	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CO544_RS03460 (702900)	702900..703235	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CO544_RS03465 (703384)	703384..704955	-	1572	WP_001273776.1	galactarate dehydratase	-
CO544_RS03470 (705330)	705330..706664	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
CO544_RS03475 (706680)	706680..707450	+	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T227523 WP_000347273.1 NZ_CP089036:c702900-702436 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T227523 NZ_CP122929:c3708032-3707634 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT227523 WP_001302819.1 NZ_CP089036:c703235-702900 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT227523 NZ_CP122929:c3708328-3708035 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7