Detailed information of TA system TA227512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4849118..4849668
• Replicon: chromosome
• Accession: NZ_CP089034
• Organism: Escherichia coli O157:H7 strain 6535NalR

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: LSI70_RS24825
• Protein ID: WP_001160975.1
• Coordinates: 4849118..4849432 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: LSI70_RS24830
• Protein ID: WP_000125566.1
• Coordinates: 4849435..4849668 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI70_RS24800 (4844793)	4844793..4846079	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
LSI70_RS24805 (4846079)	4846079..4847068	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
LSI70_RS24810 (4847065)	4847065..4847886	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
LSI70_RS24815 (4847889)	4847889..4848266	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
LSI70_RS24820 (4848273)	4848273..4849121	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
LSI70_RS24825 (4849118)	4849118..4849432	-	315	WP_001160975.1	CcdB family protein	Toxin
LSI70_RS24830 (4849435)	4849435..4849668	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LSI70_RS24835 (4849754)	4849754..4850233	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
LSI70_RS24840 (4850425)	4850425..4852287	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
LSI70_RS24845 (4852280)	4852280..4852810	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
LSI70_RS24850 (4852918)	4852918..4854249	-	1332	WP_001183177.1	MFS transporter	-
LSI70_RS24855 (4854307)	4854307..4854594	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T227512 WP_001160975.1 NZ_CP089034:c4849432-4849118 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T227512 NZ_CP122929:957040-957375 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTCG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT227512 WP_000125566.1 NZ_CP089034:c4849668-4849435 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT227512 NZ_CP122929:956792-957040 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9