Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4318051..4318669 Replicon chromosome
Accession NZ_CP089034
Organism Escherichia coli O157:H7 strain 6535NalR

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LSI70_RS22225 Protein ID WP_001291435.1
Coordinates 4318451..4318669 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LSI70_RS22220 Protein ID WP_000344800.1
Coordinates 4318051..4318425 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI70_RS22210 (4313140) 4313140..4314333 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LSI70_RS22215 (4314356) 4314356..4317505 + 3150 WP_001132452.1 efflux RND transporter permease AcrB -
LSI70_RS22220 (4318051) 4318051..4318425 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LSI70_RS22225 (4318451) 4318451..4318669 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LSI70_RS22230 (4318841) 4318841..4319392 + 552 WP_000102553.1 maltose O-acetyltransferase -
LSI70_RS22235 (4319508) 4319508..4319978 + 471 WP_000136192.1 YlaC family protein -
LSI70_RS22240 (4320142) 4320142..4321692 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LSI70_RS22245 (4321734) 4321734..4322087 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LSI70_RS22255 (4322466) 4322466..4322777 + 312 WP_000409911.1 MGMT family protein -
LSI70_RS22260 (4322810) 4322810..4323382 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227510 WP_001291435.1 NZ_CP089034:4318451-4318669 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T227510 NZ_CP122929:713586-713882 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT227510 WP_000344800.1 NZ_CP089034:4318051-4318425 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT227510 NZ_CP122929:713884-714279 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References