Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-HTH_XRE
Location 3863379..3864084 Replicon chromosome
Accession NZ_CP089034
Organism Escherichia coli O157:H7 strain 6535NalR

Toxin (Protein)


Gene name relE Uniprot ID Q8X3N0
Locus tag LSI70_RS20130 Protein ID WP_000539519.1
Coordinates 3863379..3863765 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag LSI70_RS20135 Protein ID WP_001280945.1
Coordinates 3863755..3864084 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI70_RS20110 (3859383) 3859383..3860009 + 627 WP_001295292.1 glutathione S-transferase GstB -
LSI70_RS20115 (3860006) 3860006..3861121 - 1116 WP_000554959.1 aldose sugar dehydrogenase YliI -
LSI70_RS20120 (3861232) 3861232..3861615 - 384 WP_000497137.1 biofilm formation regulator BssR -
LSI70_RS20125 (3861828) 3861828..3863153 + 1326 WP_000049367.1 30S ribosomal protein S12 methylthiotransferase RimO -
LSI70_RS20130 (3863379) 3863379..3863765 + 387 WP_000539519.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LSI70_RS20135 (3863755) 3863755..3864084 + 330 WP_001280945.1 DNA-binding transcriptional regulator Antitoxin
LSI70_RS20140 (3864154) 3864154..3865482 - 1329 WP_000086905.1 GGDEF domain-containing protein -
LSI70_RS20145 (3865490) 3865490..3867838 - 2349 WP_000950320.1 EAL domain-containing protein -
LSI70_RS20150 (3868016) 3868016..3868927 - 912 WP_001236044.1 glutathione ABC transporter permease GsiD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(1-124)

Antitoxin

Cro/C1-type helix-turn-helix domain

(53-94)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14279.42 Da        Isoelectric Point: 9.9296

>T227509 WP_000539519.1 NZ_CP089034:3863379-3863765 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Download         Length: 387 bp

>T227509 NZ_CP122929:649013-649327 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11982.08 Da        Isoelectric Point: 10.0101

>AT227509 WP_001280945.1 NZ_CP089034:3863755-3864084 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Download         Length: 330 bp

>AT227509 NZ_CP122929:649324-649740 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References