Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4589309..4589859 Replicon chromosome
Accession NZ_CP089031
Organism Escherichia coli strain 7386Nal

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag CO543_RS23190 Protein ID WP_001160975.1
Coordinates 4589309..4589623 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CO543_RS23195 Protein ID WP_000125566.1
Coordinates 4589626..4589859 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO543_RS23165 (4584984) 4584984..4586270 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CO543_RS23170 (4586270) 4586270..4587259 + 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CO543_RS23175 (4587256) 4587256..4588077 + 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CO543_RS23180 (4588080) 4588080..4588457 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CO543_RS23185 (4588464) 4588464..4589312 + 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CO543_RS23190 (4589309) 4589309..4589623 - 315 WP_001160975.1 CcdB family protein Toxin
CO543_RS23195 (4589626) 4589626..4589859 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CO543_RS23200 (4589945) 4589945..4590424 - 480 WP_000624372.1 type 3 dihydrofolate reductase -
CO543_RS23205 (4590616) 4590616..4592478 - 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
CO543_RS23210 (4592471) 4592471..4593001 - 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CO543_RS23215 (4593109) 4593109..4594440 - 1332 WP_001183177.1 MFS transporter -
CO543_RS23220 (4594498) 4594498..4594785 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T227456 WP_001160975.1 NZ_CP089031:c4589623-4589309 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T227456 NZ_CP122878:94499-94648 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT227456 WP_000125566.1 NZ_CP089031:c4589859-4589626 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT227456 NZ_CP122878:c94455-94394 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References