Detailed information of TA system TA227427

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ldrD-sokW/Ldr(toxin)
• Location: 277101..277322
• Replicon: chromosome
• Accession: NZ_CP089031
• Organism: Escherichia coli strain 7386Nal

Toxin (Protein)

• Gene name: ldrD
• Uniprot ID: S1NWX0
• Locus tag: CO543_RS01345
• Protein ID: WP_001295224.1
• Coordinates: 277215..277322 (+)
• Length: 36 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	277101..277166 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO543_RS01325 (272541)	272541..273443	+	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
CO543_RS01330 (273454)	273454..274437	+	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	-
CO543_RS01335 (274434)	274434..275438	+	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
CO543_RS01340 (275468)	275468..276739	-	1272	WP_001301684.1	aromatic amino acid transport family protein	-
- (277101)	277101..277166	-	66	NuclAT_16	-	Antitoxin
- (277101)	277101..277166	-	66	NuclAT_16	-	Antitoxin
- (277101)	277101..277166	-	66	NuclAT_16	-	Antitoxin
- (277101)	277101..277166	-	66	NuclAT_16	-	Antitoxin
- (277101)	277101..277166	-	66	NuclAT_21	-	Antitoxin
- (277101)	277101..277166	-	66	NuclAT_21	-	Antitoxin
- (277101)	277101..277166	-	66	NuclAT_21	-	Antitoxin
- (277101)	277101..277166	-	66	NuclAT_21	-	Antitoxin
CO543_RS01345 (277215)	277215..277322	+	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	Toxin
CO543_RS01350 (277409)	277409..279088	-	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
CO543_RS01355 (279085)	279085..279276	-	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
CO543_RS01360 (279273)	279273..280844	-	1572	WP_001204920.1	cellulose biosynthesis c-di-GMP-binding protein BcsE	-
CO543_RS01365 (281117)	281117..281305	+	189	WP_001063316.1	cellulose biosynthesis protein BcsR	-
CO543_RS01370 (281317)	281317..282069	+	753	Protein_273	cellulose biosynthesis protein BcsQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 3882.70 Da
• Isoelectric Point: 9.0157

>T227427 WP_001295224.1 NZ_CP089031:277215-277322 [Escherichia coli]
MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK

Nucleotide

• Length: 108 bp

>T227427 NZ_CP122872:c1132657-1132280 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGACCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTATCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT227427 NZ_CP089031:c277166-277101 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A4P7TSJ7

Antitoxin