Detailed information of TA system TA227417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2844786..2846374
• Replicon: chromosome
• Accession: NZ_CP089024
• Organism: Klebsiella pneumoniae strain 49210

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A331XLA5
• Locus tag: LRY78_RS14020
• Protein ID: WP_014907613.1
• Coordinates: 2844786..2846108 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LRY78_RS14025
• Protein ID: WP_002904790.1
• Coordinates: 2846108..2846374 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRY78_RS14005 (2839842)	2839842..2842343	+	2502	WP_014907611.1	fimbria/pilus outer membrane usher protein	-
LRY78_RS14010 (2842331)	2842331..2843335	+	1005	WP_014907612.1	fimbrial protein	-
LRY78_RS14015 (2843477)	2843477..2844769	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LRY78_RS14020 (2844786)	2844786..2846108	-	1323	WP_014907613.1	type II toxin-antitoxin system HipA family toxin	Toxin
LRY78_RS14025 (2846108)	2846108..2846374	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LRY78_RS14030 (2846612)	2846612..2846938	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
LRY78_RS14035 (2846935)	2846935..2847435	-	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LRY78_RS14040 (2847413)	2847413..2848792	-	1380	WP_004184021.1	nucleobase:cation symporter-2 family protein	-
LRY78_RS14045 (2849138)	2849138..2850292	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LRY78_RS14050 (2850273)	2850273..2851196	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49139.47 Da
• Isoelectric Point: 7.5367

>T227417 WP_014907613.1 NZ_CP089024:c2846108-2844786 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T227417 NZ_CP122868:c4566122-4565811 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT227417 WP_002904790.1 NZ_CP089024:c2846374-2846108 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT227417 NZ_CP122868:c4565810-4565520 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A331XLA5

Antitoxin

• AlphaFold DB: A0A0H3GXD8