Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 57413..58140 | Replicon | plasmid p53370-227.9 |
| Accession | NZ_CP089023 | ||
| Organism | Klebsiella pneumoniae strain 53370 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | LRZ76_RS27185 | Protein ID | WP_011251285.1 |
| Coordinates | 57829..58140 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LRZ76_RS27180 | Protein ID | WP_011251286.1 |
| Coordinates | 57413..57832 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRZ76_RS27150 (LRZ76_27150) | 52581..53051 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| LRZ76_RS27155 (LRZ76_27155) | 53364..53999 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| LRZ76_RS27160 (LRZ76_27160) | 54418..55038 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| LRZ76_RS27165 (LRZ76_27165) | 55059..55847 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| LRZ76_RS27170 (LRZ76_27170) | 55861..56226 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| LRZ76_RS27175 (LRZ76_27175) | 56298..57266 | - | 969 | WP_086642568.1 | IS5 family transposase | - |
| LRZ76_RS27180 (LRZ76_27180) | 57413..57832 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| LRZ76_RS27185 (LRZ76_27185) | 57829..58140 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| LRZ76_RS27190 (LRZ76_27190) | 58345..58782 | - | 438 | Protein_58 | DDE-type integrase/transposase/recombinase | - |
| LRZ76_RS27195 (LRZ76_27195) | 58917..59614 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| LRZ76_RS27200 (LRZ76_27200) | 59616..60065 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| LRZ76_RS27205 (LRZ76_27205) | 60082..60393 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| LRZ76_RS27210 (LRZ76_27210) | 60407..60748 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| LRZ76_RS27215 (LRZ76_27215) | 60808..61764 | + | 957 | WP_011251280.1 | DsbA family protein | - |
| LRZ76_RS27220 (LRZ76_27220) | 61881..62456 | + | 576 | WP_074424927.1 | hypothetical protein | - |
| LRZ76_RS27225 (LRZ76_27225) | 62564..63079 | + | 516 | WP_041937820.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..227890 | 227890 | |
| - | inside | IScluster/Tn | - | - | 47292..59614 | 12322 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T227403 WP_011251285.1 NZ_CP089023:c58140-57829 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T227403 NZ_CP122868:1027798-1028133 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT227403 WP_011251286.1 NZ_CP089023:c57832-57413 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT227403 NZ_CP122868:1027550-1027798 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|