227337

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-DnaT
Location 24202..24724 Replicon plasmid p56661-276.1
Accession NZ_CP089011
Organism Klebsiella pneumoniae strain 56661

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag LRZ77_RS28375 Protein ID WP_004181778.1
Coordinates 24440..24724 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A5Q9LM53
Locus tag LRZ77_RS28370 Protein ID WP_025368601.1
Coordinates 24202..24450 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ77_RS28345 (LRZ77_28340) 19518..19871 - 354 WP_004181774.1 hypothetical protein -
LRZ77_RS28350 (LRZ77_28345) 20016..21002 - 987 WP_025368599.1 hypothetical protein -
LRZ77_RS28355 (LRZ77_28350) 21336..22583 - 1248 WP_231821792.1 ATP-dependent helicase -
LRZ77_RS28360 (LRZ77_28355) 22651..23348 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
LRZ77_RS28365 (LRZ77_28360) 23283..23912 - 630 WP_231821793.1 UvrD-helicase domain-containing protein -
LRZ77_RS28370 (LRZ77_28365) 24202..24450 + 249 WP_025368601.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LRZ77_RS28375 (LRZ77_28370) 24440..24724 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LRZ77_RS28380 (LRZ77_28375) 24954..26180 + 1227 WP_025368602.1 RNA-guided endonuclease TnpB family protein -
LRZ77_RS28385 (LRZ77_28380) 26466..26891 - 426 WP_040120489.1 IS200/IS605 family transposase -
LRZ77_RS28390 (LRZ77_28385) 26928..28154 + 1227 WP_025368604.1 RNA-guided endonuclease TnpB family protein -
LRZ77_RS28395 (LRZ77_28390) 28220..29346 - 1127 Protein_34 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iucA / iucB / iucC / iucD / iutA / rmpA2 1..276100 276100


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T227337 WP_004181778.1 NZ_CP089011:24440-24724 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T227337 NZ_CP122839:213114-214439 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8906.12 Da        Isoelectric Point: 3.8206

>AT227337 WP_025368601.1 NZ_CP089011:24202-24450 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT227337 NZ_CP122839:212816-213109 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LM53

References