Detailed information of TA system TA227332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5281995..5282620
• Replicon: chromosome
• Accession: NZ_CP089001
• Organism: Klebsiella pneumoniae strain 56661

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: LRZ77_RS25910
• Protein ID: WP_002882817.1
• Coordinates: 5281995..5282378 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: LRZ77_RS25915
• Protein ID: WP_004150355.1
• Coordinates: 5282378..5282620 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ77_RS25895 (5279361)	5279361..5280263	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
LRZ77_RS25900 (5280260)	5280260..5280895	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
LRZ77_RS25905 (5280892)	5280892..5281821	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
LRZ77_RS25910 (5281995)	5281995..5282378	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRZ77_RS25915 (5282378)	5282378..5282620	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
LRZ77_RS25920 (5282825)	5282825..5283742	+	918	WP_021466676.1	alpha/beta hydrolase	-
LRZ77_RS25925 (5283756)	5283756..5284697	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
LRZ77_RS25930 (5284742)	5284742..5285179	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
LRZ77_RS25935 (5285176)	5285176..5286036	-	861	WP_004146232.1	virulence factor BrkB family protein	-
LRZ77_RS25940 (5286030)	5286030..5286629	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T227332 WP_002882817.1 NZ_CP089001:c5282378-5281995 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T227332 NZ_CP122837:3562534-3563076 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTAATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT227332 WP_004150355.1 NZ_CP089001:c5282620-5282378 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT227332 NZ_CP122837:3562239-3562550 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8

Antitoxin

• AlphaFold DB: A0A0H3GGU9