Detailed information of TA system TA022732

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2263237..2264077
• Replicon: chromosome
• Accession: NC_010498
• Organism: Escherichia coli SMS-3-5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: ECSMS35_RS11355
• Protein ID: WP_000854686.1
• Coordinates: 2263237..2263620 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0YJ34
• Locus tag: ECSMS35_RS11360
• Protein ID: WP_001360163.1
• Coordinates: 2263697..2264077 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSMS35_RS11325	2259913..2260617	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
ECSMS35_RS11330	2260902..2261120	+	219	WP_001040187.1	translation initiation factor IF-1	-
ECSMS35_RS11340	2261611..2262453	-	843	WP_001274547.1	DUF4942 domain-containing protein	-
ECSMS35_RS11345	2262538..2262735	-	198	WP_000839253.1	DUF957 domain-containing protein	-
ECSMS35_RS11350	2262752..2263240	-	489	WP_001054233.1	hypothetical protein	-
ECSMS35_RS11355	2263237..2263620	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
ECSMS35_RS11360	2263697..2264077	-	381	WP_001360163.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECSMS35_RS11365	2264088..2264771	-	684	WP_000086769.1	hypothetical protein	-
ECSMS35_RS11370	2264790..2265011	-	222	WP_000692298.1	DUF987 domain-containing protein	-
ECSMS35_RS11375	2265074..2265550	-	477	WP_001186726.1	RadC family protein	-
ECSMS35_RS11380	2265566..2266051	-	486	WP_000214307.1	antirestriction protein	-
ECSMS35_RS11385	2266143..2266961	-	819	WP_001234732.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T22732 WP_000854686.1 NC_010498:c2263620-2263237 [Escherichia coli SMS-3-5]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T22732 NC_010498:c2263620-2263237 [Escherichia coli SMS-3-5]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13950.74 Da
• Isoelectric Point: 5.0823

>AT22732 WP_001360163.1 NC_010498:c2264077-2263697 [Escherichia coli SMS-3-5]
VSDTLPGTTLPDDNKDRPWWGLPCIVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT22732 NC_010498:c2264077-2263697 [Escherichia coli SMS-3-5]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCATCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A1M0YJ34