Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42520..43045 Replicon plasmid p53322-121.6
Accession NZ_CP088989
Organism Klebsiella pneumoniae strain 53322

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag LRZ75_RS26010 Protein ID WP_025714517.1
Coordinates 42740..43045 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LRZ75_RS26005 Protein ID WP_001568025.1
Coordinates 42520..42738 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ75_RS25970 (LRZ75_25970) 37771..38529 + 759 Protein_44 putrescine ABC transporter permease SapB -
LRZ75_RS25975 (LRZ75_25975) 38572..39276 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LRZ75_RS25980 (LRZ75_25980) 39339..40166 - 828 Protein_46 IS4-like element ISVsa5 family transposase -
LRZ75_RS25985 (LRZ75_25985) 40272..41216 - 945 Protein_47 AAA family ATPase -
LRZ75_RS25990 (LRZ75_25990) 41290..41706 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
LRZ75_RS25995 (LRZ75_25995) 41703..41933 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
LRZ75_RS26000 (LRZ75_26000) 41890..42350 + 461 Protein_50 hypothetical protein -
LRZ75_RS26005 (LRZ75_26005) 42520..42738 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LRZ75_RS26010 (LRZ75_26010) 42740..43045 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
LRZ75_RS26015 (LRZ75_26015) 43214..43609 + 396 WP_017899885.1 hypothetical protein -
LRZ75_RS26020 (LRZ75_26020) 43636..43950 + 315 WP_053389906.1 hypothetical protein -
LRZ75_RS26025 (LRZ75_26025) 43961..44977 + 1017 WP_017899884.1 hypothetical protein -
LRZ75_RS26030 (LRZ75_26030) 45175..45969 + 795 WP_004197635.1 site-specific integrase -
LRZ75_RS26035 (LRZ75_26035) 46457..46759 - 303 WP_071571079.1 hypothetical protein -
LRZ75_RS26040 (LRZ75_26040) 46756..47382 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / blaDHA-1 / qnrB4 - 1..121561 121561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T227257 WP_025714517.1 NZ_CP088989:42740-43045 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T227257 NZ_CP122816:2552059-2553381 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT227257 WP_001568025.1 NZ_CP088989:42520-42738 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT227257 NZ_CP122816:2551793-2552059 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References