Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 35732..36468 Replicon plasmid unnamed1
Accession NZ_CP088979
Organism Klebsiella pneumoniae strain KP015

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LST75_RS26020 Protein ID WP_003026803.1
Coordinates 35732..36214 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LST75_RS26025 Protein ID WP_003026799.1
Coordinates 36202..36468 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LST75_RS25990 (LST75_25940) 31136..32101 + 966 WP_004118146.1 DMT family transporter -
LST75_RS25995 (LST75_25945) 32169..32297 - 129 Protein_33 transcriptional regulator -
LST75_RS26000 (LST75_25950) 32357..32971 - 615 WP_023316428.1 hypothetical protein -
LST75_RS26005 (LST75_25955) 33152..33988 - 837 WP_032409249.1 EAL domain-containing protein -
LST75_RS26010 (LST75_25960) 34052..34567 - 516 Protein_36 CSS-motif domain-containing protein -
LST75_RS26015 (LST75_25965) 35209..35466 + 258 WP_023292103.1 hypothetical protein -
LST75_RS26020 (LST75_25970) 35732..36214 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LST75_RS26025 (LST75_25975) 36202..36468 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LST75_RS26030 (LST75_25980) 36692..36944 + 253 Protein_40 transposase -
LST75_RS26035 (LST75_25985) 37103..37408 - 306 WP_227522558.1 CPBP family intramembrane metalloprotease -
LST75_RS26040 (LST75_25990) 38936..39391 + 456 Protein_42 transposase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..144296 144296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T227191 WP_003026803.1 NZ_CP088979:c36214-35732 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T227191 NZ_CP122768:274725-274832 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGATCATTGCTAGCATTCTTGCCAGTCTGAT
CGTGAACTCGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT227191 WP_003026799.1 NZ_CP088979:c36468-36202 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT227191 NZ_CP122768:c274667-274613 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References