Detailed information of TA system TA227151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2226457..2227047
• Replicon: chromosome
• Accession: NZ_CP088925
• Organism: Rhodanobacter denitrificans strain FW104-MT042

Toxin (Protein)

• Gene name: graT
• Uniprot ID: I4WLY1
• Locus tag: LRK55_RS10125
• Protein ID: WP_007512781.1
• Coordinates: 2226457..2226738 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK55_RS10130
• Protein ID: WP_007512782.1
• Coordinates: 2226748..2227047 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK55_RS10115 (LRK55_10115)	2223007..2225082	+	2076	WP_235644107.1	N-6 DNA methylase	-
LRK55_RS10120 (LRK55_10120)	2225079..2226377	+	1299	WP_235644108.1	restriction endonuclease subunit S	-
LRK55_RS10125 (LRK55_10125)	2226457..2226738	+	282	WP_007512781.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK55_RS10130 (LRK55_10130)	2226748..2227047	+	300	WP_007512782.1	HigA family addiction module antitoxin	Antitoxin
LRK55_RS10135 (LRK55_10135)	2227101..2230340	+	3240	WP_235644109.1	type I restriction endonuclease subunit R	-
LRK55_RS10140 (LRK55_10140)	2230403..2230567	+	165	Protein_1993	glycerophosphodiester phosphodiesterase family protein	-
LRK55_RS10145 (LRK55_10145)	2230654..2231259	-	606	WP_235644110.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10798.46 Da
• Isoelectric Point: 10.3017

>T227151 WP_007512781.1 NZ_CP088925:2226457-2226738 [Rhodanobacter denitrificans]
MTIQSFRCKDTQALFERRRVRRFLVIEAAARRKLELLNAATQLDYLRVPPGNRLEALAGDRKGQHSIRVNDQFRLCFVWT
ADGPGLVEIVDYH

Nucleotide

• Length: 282 bp

>T227151 NZ_CP122727:1840273-1840527 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATATCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.79 Da
• Isoelectric Point: 7.9969

>AT227151 WP_007512782.1 NZ_CP088925:2226748-2227047 [Rhodanobacter denitrificans]
MPRKLPPIHPGEILREEYMLPLELSSNALARALEVTPARINEIVRERRGISADTALRLARYFGNDAQSWLNLQQRYDIEC
AQDSIGGALRKITPYQAAA

Nucleotide

• Length: 300 bp

>AT227151 NZ_CP122727:1840025-1840276 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: I4WLY1