Detailed information of TA system TA227149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HTH_37(antitoxin)
• Location: 561595..562223
• Replicon: chromosome
• Accession: NZ_CP088925
• Organism: Rhodanobacter denitrificans strain FW104-MT042

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LRK55_RS02615
• Protein ID: WP_007513282.1
• Coordinates: 562047..562223 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LRK55_RS02610
• Protein ID: WP_027484763.1
• Coordinates: 561595..562014 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK55_RS02600 (LRK55_02600)	557589..560099	+	2511	WP_235644758.1	DEAD/DEAH box helicase family protein	-
LRK55_RS02605 (LRK55_02605)	560163..561212	+	1050	WP_027484764.1	nuclease-related domain-containing protein	-
LRK55_RS02610 (LRK55_02610)	561595..562014	-	420	WP_027484763.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LRK55_RS02615 (LRK55_02615)	562047..562223	-	177	WP_007513282.1	type II toxin-antitoxin system HicA family toxin	Toxin
LRK55_RS02620 (LRK55_02620)	562402..563064	+	663	WP_027484762.1	DUF502 domain-containing protein	-
LRK55_RS02625 (LRK55_02625)	563149..564960	+	1812	WP_235644760.1	M14 family metallopeptidase	-
LRK55_RS02630 (LRK55_02630)	565079..567202	+	2124	WP_027489900.1	M13-type metalloendopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6834.93 Da
• Isoelectric Point: 11.0888

>T227149 WP_007513282.1 NZ_CP088925:c562223-562047 [Rhodanobacter denitrificans]
MKYSEFQRWLRQQGVSFEPGKGSHQLARYRGRTSVFPFHGSKEMPEPLRKKILKDLGL

Nucleotide

• Length: 177 bp

>T227149 NZ_CP122727:1010637-1010972 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15454.93 Da
• Isoelectric Point: 8.0281

>AT227149 WP_027484763.1 NZ_CP088925:c562014-561595 [Rhodanobacter denitrificans]
MKFPVKLTRADSVVMVTCPDLPEFSSVGDTEGEALREAVDGIETTLQAYMQDRRDIPPPGARKRGQRLVELPPLAVAKLG
LYRAMREQGLRKADLARRLDVHLPQVDRLLDLRHSSKLEQVEAALNALGYRIELAVLAA

Nucleotide

• Length: 420 bp

>AT227149 NZ_CP122727:1010389-1010637 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA