Detailed information of TA system TA227127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 700766..701356
• Replicon: chromosome
• Accession: NZ_CP088919
• Organism: Rhodanobacter denitrificans strain DSM-24678

Toxin (Protein)

• Gene name: graT
• Uniprot ID: I4WLY1
• Locus tag: LRK24_RS03085
• Protein ID: WP_007512781.1
• Coordinates: 700766..701047 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK24_RS03090
• Protein ID: WP_007512782.1
• Coordinates: 701057..701356 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK24_RS03075 (LRK24_03075)	697463..699436	+	1974	WP_007512779.1	class I SAM-dependent DNA methyltransferase	-
LRK24_RS03080 (LRK24_03080)	699433..700686	+	1254	WP_015448950.1	restriction endonuclease subunit S	-
LRK24_RS03085 (LRK24_03085)	700766..701047	+	282	WP_007512781.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK24_RS03090 (LRK24_03090)	701057..701356	+	300	WP_007512782.1	HigA family addiction module antitoxin	Antitoxin
LRK24_RS03095 (LRK24_03095)	701410..704649	+	3240	WP_007512783.1	type I restriction endonuclease subunit R	-
LRK24_RS03100 (LRK24_03100)	704712..704867	+	156	Protein_612	glycerophosphodiester phosphodiesterase family protein	-
LRK24_RS03105 (LRK24_03105)	704963..705547	-	585	WP_236100418.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10798.46 Da
• Isoelectric Point: 10.3017

>T227127 WP_007512781.1 NZ_CP088919:700766-701047 [Rhodanobacter denitrificans]
MTIQSFRCKDTQALFERRRVRRFLVIEAAARRKLELLNAATQLDYLRVPPGNRLEALAGDRKGQHSIRVNDQFRLCFVWT
ADGPGLVEIVDYH

Nucleotide

• Length: 282 bp

>T227127 NZ_CP122722:c2011343-2010969 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.79 Da
• Isoelectric Point: 7.9969

>AT227127 WP_007512782.1 NZ_CP088919:701057-701356 [Rhodanobacter denitrificans]
MPRKLPPIHPGEILREEYMLPLELSSNALARALEVTPARINEIVRERRGISADTALRLARYFGNDAQSWLNLQQRYDIEC
AQDSIGGALRKITPYQAAA

Nucleotide

• Length: 300 bp

>AT227127 NZ_CP122722:c2011801-2011433 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCACCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTAACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: I4WLY1