Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacT-ataR/DUF1778(antitoxin)
Location 28611..29368 Replicon plasmid pESI
Accession NZ_CP088902
Organism Salmonella enterica subsp. enterica serovar Muenchen strain 180135033

Toxin (Protein)


Gene name tacT Uniprot ID A0A241PXX4
Locus tag LRP35_RS23895 Protein ID WP_023994162.1
Coordinates 28886..29368 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A727Z1I8
Locus tag LRP35_RS23890 Protein ID WP_001195098.1
Coordinates 28611..28895 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRP35_RS23845 (LRP35_23850) 24452..24598 - 147 Protein_20 IS66 family insertion sequence element accessory protein TnpB -
LRP35_RS23850 (LRP35_23855) 24585..24914 - 330 WP_023994169.1 hypothetical protein -
LRP35_RS23855 (LRP35_23860) 25038..25891 + 854 Protein_22 site-specific tyrosine recombinase XerC -
LRP35_RS23860 (LRP35_23865) 25860..26147 + 288 WP_031619580.1 damage-inducible protein J -
LRP35_RS23865 (LRP35_23870) 26144..26449 + 306 WP_023994167.1 type II toxin-antitoxin system RelE/ParE family toxin -
LRP35_RS23870 (LRP35_23875) 27199..27462 + 264 WP_023994165.1 DUF977 family protein -
LRP35_RS23875 (LRP35_23880) 27488..27823 + 336 WP_023994164.1 hypothetical protein -
LRP35_RS23880 (LRP35_23885) 28045..28242 - 198 Protein_27 PIN domain-containing protein -
LRP35_RS23885 (LRP35_23890) 28366..28455 + 90 WP_071790431.1 helix-turn-helix domain-containing protein -
LRP35_RS23890 (LRP35_23895) 28611..28895 + 285 WP_001195098.1 DUF1778 domain-containing protein Antitoxin
LRP35_RS23895 (LRP35_23900) 28886..29368 + 483 WP_023994162.1 GNAT family N-acetyltransferase Toxin
LRP35_RS23900 (LRP35_23905) 29683..30109 + 427 Protein_31 transposase -
LRP35_RS23905 (LRP35_23910) 30426..30635 + 210 WP_023994160.1 hemolysin expression modulator Hha -
LRP35_RS23910 (LRP35_23915) 30709..31061 - 353 Protein_33 transposase -
LRP35_RS23915 (LRP35_23920) 31288..32261 + 974 Protein_34 IS256 family transposase -
LRP35_RS23920 (LRP35_23925) 32263..32505 + 243 WP_023994156.1 hypothetical protein -
LRP35_RS23925 (LRP35_23930) 32576..33532 + 957 WP_023994155.1 Rpn family recombination-promoting nuclease/putative transposase -
LRP35_RS23930 (LRP35_23935) 33591..33803 + 213 WP_023994154.1 hypothetical protein -
LRP35_RS23935 (LRP35_23940) 33845..34096 - 252 WP_023994153.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / qacE / sul1 / tet(A) / dfrA14 faeC / faeD / faeE / faeF / faeH / faeI / fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS 1..285167 285167
- inside IScluster/Tn - - 23156..35660 12504


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(31-149)

Antitoxin

Vibrio phage ICP1, Orf50

(13-88)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17971.54 Da        Isoelectric Point: 6.8521

>T227117 WP_023994162.1 NZ_CP088902:28886-29368 [Salmonella enterica subsp. enterica serovar Muenchen]
MEISVTAPELLNEEHYLQQFDCGNDVLSDWLRRRAMKNQYLNASRTFVICPEGTKRVVGYYSIATGSVSHASLGRSLRQN
MPDPVPVVLLGRLAVDECTQGHSFGKWLLNDAVTRVSNLADQVGIKAIMVHAIDEQAKTFYEYFGFVQSPIAPNTLFYKI

Download         Length: 483 bp

>T227117 NZ_CP122722:305366-305893 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10964.52 Da        Isoelectric Point: 9.6539

>AT227117 WP_001195098.1 NZ_CP088902:28611-28895 [Salmonella enterica subsp. enterica serovar Muenchen]
MQTTIRKSVRNKQINIRATDEERAVIDYAASLVSKNRTDFIIEKAVSEAQNIILDQRVFVLDDARYQAFIKQLEAPVQNT
EGRQRLMDVKPEWK

Download         Length: 285 bp

>AT227117 NZ_CP122722:305093-305359 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241PXX4

Antitoxin

Source ID Structure
AlphaFold DB A0A727Z1I8

References