Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 198298..198938 Replicon plasmid pBb323S2b
Accession NZ_CP088281
Organism Bradyrhizobium barranii subsp. barranii strain 323S2

Toxin (Protein)


Gene name vapC Uniprot ID A0A7Z0QNE7
Locus tag G6321_RS54635 Protein ID WP_035696746.1
Coordinates 198298..198684 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7Z0QMX7
Locus tag G6321_RS54640 Protein ID WP_028153009.1
Coordinates 198684..198938 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6321_RS54620 (G6321_00054620) 194332..195609 + 1278 WP_050995298.1 plasmid replication protein RepC -
G6321_RS54625 (G6321_00054625) 195767..196672 + 906 WP_166354321.1 DUF3991 and toprim domain-containing protein -
G6321_RS54630 (G6321_00054630) 197222..198295 - 1074 WP_166354319.1 tyrosine-type recombinase/integrase -
G6321_RS54635 (G6321_00054635) 198298..198684 - 387 WP_035696746.1 type II toxin-antitoxin system VapC family toxin Toxin
G6321_RS54640 (G6321_00054640) 198684..198938 - 255 WP_028153009.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
G6321_RS54645 (G6321_00054645) 199049..199456 - 408 WP_166354317.1 type II toxin-antitoxin system VapC family toxin -
G6321_RS54650 (G6321_00054650) 199456..199710 - 255 WP_038381008.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
G6321_RS54655 (G6321_00054655) 200041..200961 + 921 WP_224518052.1 DUF1403 family protein -
G6321_RS54660 (G6321_00054660) 200963..201694 + 732 WP_224518054.1 SMC-Scp complex subunit ScpB -
G6321_RS54665 (G6321_00054665) 201800..202918 + 1119 WP_224518056.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..294915 294915


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 13704.59 Da        Isoelectric Point: 5.5902

>T227073 WP_035696746.1 NZ_CP088281:c198684-198298 [Bradyrhizobium barranii subsp. barranii]
MVIDSSAILAIFLNEPEASEFEHRIAADPVRLISAATVLETGIGIESKLGDGGGAELDLWLHKVGVEIVPVTAGHADQAR
RAWRRYGKGRHPAKLNFGDCFSYALAALTGEPLLFKGSDFSQTDIKAA

Download         Length: 387 bp

>T227073 NZ_CP122712:c1017361-1016954 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9317.71 Da        Isoelectric Point: 4.8660

>AT227073 WP_028153009.1 NZ_CP088281:c198938-198684 [Bradyrhizobium barranii subsp. barranii]
MALSIKDPETEKLARTLAERTGETITVATRRAIEDRLRRIGADATKAALIEDMEAIRRRLAALPVLDPRSMDDILGYDEN
GLPV

Download         Length: 255 bp

>AT227073 NZ_CP122712:c1017608-1017342 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z0QNE7

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z0QMX7

References