Detailed information of TA system TA226837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 23472..24097
• Replicon: plasmid pB-4548_1
• Accession: NZ_CP088143
• Organism: Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4548

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LRM93_RS22720
• Protein ID: WP_000911323.1
• Coordinates: 23699..24097 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8F2UQ48
• Locus tag: LRM93_RS22715
• Protein ID: WP_000450263.1
• Coordinates: 23472..23699 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM93_RS22715 (LRM93_22715)	23472..23699	+	228	WP_000450263.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LRM93_RS22720 (LRM93_22720)	23699..24097	+	399	WP_000911323.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LRM93_RS22725 (LRM93_22725)	24106..26340	-	2235	WP_000009313.1	type IV conjugative transfer system coupling protein TraD	-
LRM93_RS22730 (LRM93_22730)	26620..27231	-	612	WP_232520941.1	hypothetical protein	-
LRM93_RS22735 (LRM93_22735)	27351..28082	-	732	WP_000782437.1	conjugal transfer complement resistance protein TraT	-
LRM93_RS22740 (LRM93_22740)	28181..28492	+	312	WP_000522257.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefB / pefA / pefC / pefD / rck / spvB / spvC / fdeC	1..92906	92906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14885.16 Da
• Isoelectric Point: 9.2199

>T226837 WP_000911323.1 NZ_CP088143:23699-24097 [Salmonella enterica subsp. enterica serovar Gallinarum]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSRMPERNLAVIEGFVSRLDVLDYDAPAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVGGLRTEDWS

Nucleotide

• Length: 399 bp

>T226837 NZ_CP122634:c4547460-4547173 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAACAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8532.53 Da
• Isoelectric Point: 4.7513

>AT226837 WP_000450263.1 NZ_CP088143:23472-23699 [Salmonella enterica subsp. enterica serovar Gallinarum]
METSVFLSNRSQAVRLPKAVALPEHVKRVEVIAVGRTRIITPAGESWDEWFDGNNVSADFMDNREQPSVQERESF

Nucleotide

• Length: 228 bp

>AT226837 NZ_CP122634:c4547186-4546884 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA