Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3714289..3714968 | Replicon | chromosome |
| Accession | NZ_CP088131 | ||
| Organism | Escherichia coli strain 81 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | S1FHS4 |
| Locus tag | LRM32_RS18195 | Protein ID | WP_000854680.1 |
| Coordinates | 3714627..3714968 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | S1EWR7 |
| Locus tag | LRM32_RS18190 | Protein ID | WP_000070396.1 |
| Coordinates | 3714289..3714606 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRM32_RS18145 (3709727) | 3709727..3710548 | + | 822 | WP_000197387.1 | DUF932 domain-containing protein | - |
| LRM32_RS18150 (3710765) | 3710765..3711466 | + | 702 | WP_000189411.1 | WYL domain-containing protein | - |
| LRM32_RS18155 (3711507) | 3711507..3711743 | + | 237 | WP_001144031.1 | protein YpjK | - |
| LRM32_RS18160 (3711743) | 3711743..3712186 | + | 444 | WP_000649865.1 | hypothetical protein | - |
| LRM32_RS18165 (3712209) | 3712209..3712676 | + | 468 | WP_001547765.1 | protein YkfB | - |
| LRM32_RS18170 (3712753) | 3712753..3712992 | + | 240 | WP_106642549.1 | DUF905 domain-containing protein | - |
| LRM32_RS18175 (3713090) | 3713090..3713548 | + | 459 | WP_106735805.1 | antirestriction protein | - |
| LRM32_RS18180 (3713564) | 3713564..3714040 | + | 477 | WP_000811693.1 | RadC family protein | - |
| LRM32_RS18185 (3714049) | 3714049..3714270 | + | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| LRM32_RS18190 (3714289) | 3714289..3714606 | + | 318 | WP_000070396.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| LRM32_RS18195 (3714627) | 3714627..3714968 | + | 342 | WP_000854680.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| LRM32_RS18200 (3715634) | 3715634..3715843 | + | 210 | Protein_3576 | integrase core domain-containing protein | - |
| LRM32_RS18205 (3715885) | 3715885..3716628 | - | 744 | WP_032181112.1 | AraC family transcriptional regulator | - |
| LRM32_RS18210 (3717029) | 3717029..3718009 | - | 981 | WP_000019445.1 | IS5-like element ISKpn26 family transposase | - |
| LRM32_RS18215 (3718080) | 3718080..3718463 | + | 384 | Protein_3579 | phage tail protein | - |
| LRM32_RS18220 (3718514) | 3718514..3719211 | + | 698 | WP_103215986.1 | IS1-like element IS1A family transposase | - |
| LRM32_RS18225 (3719222) | 3719222..3719377 | - | 156 | WP_060617805.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12892.95 Da Isoelectric Point: 9.6543
>T226734 WP_000854680.1 NZ_CP088131:3714627-3714968 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T226734 NZ_CP122617:4164096-4164353 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11982.62 Da Isoelectric Point: 7.0270
>AT226734 WP_000070396.1 NZ_CP088131:3714289-3714606 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT226734 NZ_CP122617:4164365-4164910 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|