Detailed information of TA system TA226638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1845169..1845799
• Replicon: chromosome
• Accession: NZ_CP088113
• Organism: Sinorhizobium meliloti strain RRI128

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: SmelRRI128_RS09095
• Protein ID: WP_003530010.1
• Coordinates: 1845169..1845357 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: SmelRRI128_RS09100
• Protein ID: WP_027989369.1
• Coordinates: 1845362..1845799 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SmelRRI128_RS09080 (SmelRRI128_09080)	1843071..1844042	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
SmelRRI128_RS09090 (SmelRRI128_09090)	1844223..1844992	-	770	Protein_1785	Stf0 family sulphotransferase	-
SmelRRI128_RS09095 (SmelRRI128_09095)	1845169..1845357	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
SmelRRI128_RS09100 (SmelRRI128_09100)	1845362..1845799	+	438	WP_027989369.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SmelRRI128_RS09110 (SmelRRI128_09110)	1846142..1847059	-	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
SmelRRI128_RS09115 (SmelRRI128_09115)	1847236..1847985	-	750	WP_027989370.1	NUDIX hydrolase	-
SmelRRI128_RS09120 (SmelRRI128_09120)	1847988..1849094	-	1107	WP_027989371.1	endonuclease	-
SmelRRI128_RS09125 (SmelRRI128_09125)	1849245..1849688	-	444	WP_027989372.1	TIGR02594 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T226638 WP_003530010.1 NZ_CP088113:1845169-1845357 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T226638 NZ_CP122587:c5302636-5302253 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 15534.34 Da
• Isoelectric Point: 4.3251

>AT226638 WP_027989369.1 NZ_CP088113:1845362-1845799 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDVENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSA

Nucleotide

• Length: 438 bp

>AT226638 NZ_CP122587:c5302878-5302636 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: H0G1A3